Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
| Autor: | Marcus V. G. Lacerda, Bernardo S. Franklin, Barbara L. F. Vitorino, Marina L. S. Santos, Armando de Menezes-Neto, Luzia H. Carvalho, Fernanda Magalhães Freire Campos, Cor Jesus Fernandes Fontes, Cristiana Ferreira Alves de Brito, Helena Cristina Cardoso Coelho |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Plasmodium Plasmodium vivax lcsh:Medicine Blood plasma Nucleic Acids Pathology Plasmodium Vivax Young adult lcsh:Science Immune Response Multidisciplinary Genome biology Middle Aged Parasitic diseases Infectious Diseases Medicine Female Brazil Research Article Adult Platelets Immunology Malignancy Antimalarials Young Adult Diagnostic Medicine Malarial parasites parasitic diseases medicine Parasitic Diseases Malaria Vivax Humans Telomerase reverse transcriptase Biology Aged Base Sequence lcsh:R Tropical Diseases (Non-Neglected) medicine.disease biology.organism_classification Thrombocytopenia Malaria Vivax malaria Nucleic acid lcsh:Q Biomarkers General Pathology |
| Zdroj: | Repositório Institucional da FIOCRUZ (ARCA) Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ PLoS ONE PLoS ONE, Vol 6, Iss 5, p e19842 (2011) |
| Popis: | Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Universidade Federal de Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, Brazil Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brazil Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil Background: Given the increasing evidence of Plasmodium vivax infections associated with severe and fatal disease, the identification of sensitive and reliable markers for vivax severity is crucial to improve patient care. Circulating nucleic acids (CNAs) have been increasingly recognized as powerful diagnostic and prognostic tools for various inflammatory diseases and tumors as their plasma concentrations increase according to malignancy. Given the marked inflammatory status of P. vivax infection, we investigated here the usefulness of CNAs as biomarkers for malaria morbidity. Methods and Findings: CNAs levels in plasma from twenty-one acute P. vivax malaria patients from the Brazilian Amazon and 14 malaria non-exposed healthy donors were quantified by two different methodologies: amplification of the human telomerase reverse transcriptase (hTERT) genomic sequence by quantitative real time PCR (qPCR), and the fluorometric dsDNA quantification by Pico Green. CNAs levels were significantly increased in plasma from P. vivax patients as compared to healthy donors (p |
| Databáze: | OpenAIRE |
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