Asteraceae Artemisia campestris and Artemisia herba-alba Essential Oils Trigger Apoptosis and Cell Cycle Arrest in Leishmania infantum Promastigotes

Autor: Meriam Haoues, H. Karoui, Khadija Essafi-Benkhadir, Imen Bassoumi Jamoussi, Noura Neffati, Mohamed Boussaid, Zohra Aloui, Chokri Messaoud, Ikram Guizani
Přispěvatelé: Laboratoire d'Epidémiologie Moléculaire et de Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04), Université de Tunis El Manar (UTM)-Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), Unite Ressources Phytogenet & Biotechnol Vegetale, Institut National des Sciences Appliquées et de Technologie - Carthage (INSAT Carthage), Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, This research was supported by a grant of the Ministry of Higher Education and Scientific Research (Laboratoire d’Epidémiologie Moléculaire et Pathologie Expérimentale Appliquée aux Maladies Infectieuses LR11IPT04) and the National Institute of Applied Science and Technology (Unité Ressources Phytogénétiques et Biotechnologie Végétale)., The authors are thankful to Dr. Makram Essafi for helping with fluorescence reading and to Mrs. Safa Tarhouni for helping with GraphPad Prism software. The authors want to address special thanks to Dr. Najet Srairi-Abid for critical reading and editing of the revised version of this manuscript.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine
Evidence-Based Complementary and Alternative Medicine, Hindawi Publishing Corporation, 2016, 2016, pp.9147096. ⟨10.1155/2016/9147096⟩
Evidence-Based Complementary and Alternative Medicine, Vol 2016 (2016)
ISSN: 1741-427X
1741-4288
Popis: We report the chemical composition and anti-Leishmaniaand antioxidant activity ofArtemisia campestrisL. andArtemisia herba-albaAsso. essential oils (EOs). Our results showed that these extracts exhibit different antioxidant activities according to the used assay. The radical scavenging effects determined by DPPH assay were of IC50= 3.3 mg/mL and IC50= 9.1 mg/mL forArtemisia campestrisandArtemisia herba-albaessential oils, respectively. However, antioxidant effects of both essential oils, determined by ferric-reducing antioxidant power (FRAP) assay, were in the same range (2.3 and 2.97 mg eq EDTA/g EO, resp.), while theArtemisia herba-albaessential oil showed highest chelating activity of Fe2+ions (27.48 mM Fe2+). Interestingly, we showed that both EOs possess dose-dependent activity againstLeishmania infantumpromastigotes with IC50values of 68 μg/mL and 44 μg/mL forA. herba-albaandA. campestris, respectively. We reported, for the first time, that antileishmanial activity of both EOs was mediated by cell apoptosis induction and cell cycle arrest at the sub-G0/G1 phase. All our results showed that EOs fromA. herba-albaandA. campestrisplants are promising candidates as anti-Leishmaniamedicinal products.
Databáze: OpenAIRE
Externí odkaz: