Virus (Herpes Simplex, Vaccinia) Studies in Embryonated Eggs with Radioactive Phosphorus
| Autor: | Oscar C. Liu, Harvey Blank, Ben Kaneda |
|---|---|
| Rok vydání: | 1952 |
| Předmět: |
Amniotic fluid
Inoculation Embryonated Herpes Simplex Phosphorus Vaccinia virus Biology General Biochemistry Genetics and Molecular Biology Virus Microbiology body regions Radioactivity medicine.anatomical_structure Streptomycin Viruses embryonic structures Toxicity Vaccinia medicine Phosphorus Dietary Yolk sac Phosphorus Radioisotopes Incubation medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 79:404-409 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | Conclusions1. The following observations were made on basic methods of using P32 in biological studies: a. P32 as received from Oak Ridge is usually sterile but penicillin and streptomycin can be added before direct inoculation into eggs to obviate autoclaving procedures potentially dangerous to laboratory personnel, b. Glassware and needles used for virus work can be decontaminated of P32 in 2-4 days with hot sodium metaphosphate (CalgoniteR) solutions, c. Simple methods of handling and storing millicurie amounts of P32 in biological materials are described. 2. Radioactive phosphorus is of relatively low toxicity for chick embryos; 500-1000 μc per egg is not lethal in 72 hours. 3. P32 injected into the yolk sac appears in a 100-fold greater concentration in the allantoic fluid than in the amniotic fluid in 24-96 hours. 4. The partition of P32 in the egg fluids is the same for 8-or 9- or 10-day-old embryos with periods of incubation of 24-96 hours following inoculation. 5. Following infection with herpes ... |
| Databáze: | OpenAIRE |
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