Ulvan Activates Chicken Heterophils and Monocytes Through Toll-Like Receptor 2 and Toll-Like Receptor 4
| Autor: | Matthieu Le Goff, Christelle Gouin, Frédérick Bussy, Nathalie Guriec, Olivier Mathiaud, Pi Nyvall Collén, Benoit Quero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy Immunology heterophil Inflammation Monocytes Avian Proteins 03 medical and health sciences 0302 clinical medicine Immune system ulvan Polysaccharides In vivo TLR medicine Animals Immunology and Allergy Receptor Original Research Toll-like receptor Chemistry avian Monocyte Toll-Like Receptor 2 Cell biology Toll-Like Receptor 4 TLR2 030104 developmental biology medicine.anatomical_structure inflammation monocyte TLR4 Cytokines medicine.symptom lcsh:RC581-607 Chickens 030215 immunology |
| Zdroj: | Frontiers in Immunology, Vol 9 (2018) Frontiers in Immunology |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2018.02725/full |
| Popis: | Responsiveness to invasive pathogens, clearance via the inflammatory response, and activation of appropriate acquired responses are all coordinated by innate host defenses. Toll-like receptor (TLR) ligands are potent immune-modulators with profound effects on the generation of adaptive immune responses. This property is being exploited in TLR-based vaccines and therapeutic agents in chickens. However, for administering the TLR agonist, all previous studies used in ovo, intra-muscular or intra-venous routes that cannot be performed in usual farming conditions, thus highlighting the need for TLR ligands that display systemic immune effects when given orally (per os). Here we have demonstrated that an ulvan extract of Ulva armoricana is able to activate avian heterophils and monocytes in vitro. Using specific inhibitors, we have evidenced that ulvan may be a new ligand for TLR2 and TLR4; and that they regulate heterophil activation in slightly different manner. Moreover, activation of heterophils as well as of monocytes leads to release pro-inflammatory cytokines, including interleukin1-β, interferon α and interferon γ, through pathways that we partly identified. Finally, when given per os to animals ulvan induces heterophils and monocytes to be activated in vivo thus leading to a transient release of pro-inflammatory cytokines with plasma concentrations returning toward baseline levels at day 3. |
| Databáze: | OpenAIRE |
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