Study in pig coronary smooth muscle cell subcellular fractions of the activity of various enzymes involved in lipid metabolism and of the beta-receptor adenylate cyclase couple
| Autor: | Patrick Nef, Elisabeth Imesch, Jean-Paul Giacobino |
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| Rok vydání: | 1984 |
| Předmět: |
Physiology
Swine Adenylate kinase Biology In Vitro Techniques Biochemistry Cyclase Muscle Smooth Vascular chemistry.chemical_compound Receptors Adrenergic beta Animals Molecular Biology chemistry.chemical_classification Guanylyl Imidodiphosphate Cell Membrane Fatty acid Lipid metabolism General Medicine Metabolism Lipid Metabolism Coronary Vessels Kinetics Enzyme chemistry Dihydroalprenolol Sodium Fluoride Cyclase activity Adenylyl Cyclases Subcellular Fractions |
| Zdroj: | Comparative biochemistry and physiology. B, Comparative biochemistry. 77(3) |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. Pig coronary smooth muscle cells contain a highly active fatty acid β-oxidation system as well as significant activities of other enzymes involved in lipid metabolism, i.e. palmitoyl-CoA synthetase, glycerophosphate acyl transferase, acetyl-CoA carboxylase and fatty acid synthetase. 2. 2. Purified plasma membranes of pig coronary smooth muscle cells contain stereospecific (−)-[3H]dihydroalprenolol binding sites of high affinity (Kd = 5.4nM) and characteristic of the β1 subtype. They also contain an adenylate cyclase activity which is stimulated by 5′-guanylyl-imidodiphosphate (Gpp(NH)p) and, to a lesser degree, by (–)-isoproterenol. |
| Databáze: | OpenAIRE |
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