Particulate systems based on pectin/chitosan association for the delivery of manuka honey components and platelet lysate in chronic skin ulcers
| Autor: | Maria Daglia, Maria Cristina Bonferoni, Cesare Perotti, Luciana Gioglio, Marika Tenci, Antonia Icaro Cornaglia, Cinzia Boselli, Giuseppina Sandri, Silvia Rossi, Arianna Di Lorenzo, Franca Ferrari, Carla Caramella |
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| Přispěvatelé: | Tenci, Marika, Rossi, SILVIA STEFANIA, Bonferoni, MARIA CRISTINA, Sandri, Giuseppina, Boselli, Cinzia, DI LORENZO, Arianna, Daglia, Maria, ICARO CORNAGLIA, Antonia, Gioglio, Luciana, C., Perotti, Caramella, CARLA MARCELLA, Ferrari, Franca |
| Rok vydání: | 2016 |
| Předmět: |
Blood Platelets
Male medicine.medical_specialty food.ingredient Pectin Pharmaceutical Science chemistry.chemical_element 02 engineering and technology Calcium 030226 pharmacology & pharmacy Manuka Honey Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food In vivo Manuka honey Skin Ulcer medicine Platelet lysate Animals Humans Particle Size Rats Wistar Cells Cultured Cell Proliferation Wound Healing Chromatography Methylglyoxal Honey Fibroblasts 021001 nanoscience & nanotechnology Surgery Rats Leptospermum chemistry Polymeric particle Pectins Particle size Plant Preparations 0210 nano-technology Gels |
| Zdroj: | International journal of pharmaceutics. 509(1-2) |
| ISSN: | 1873-3476 |
| Popis: | The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components and platelet lysate (PL) in chronic skin ulcers. In particular pectin (PEC)/chitosan (CS) particles were prepared by ionotropic gelation in the presence of calcium chloride and subsequently characterized for particle size, hydration properties and mechanical resistance. Different experimental conditions (calcium chloride and CS concentrations; rest time in the cationic solution) were considered in order to obtain particles characterized by optimal size, hydration properties and mechanical resistance. Two different fractions of MH were examined: one (Fr1), rich in methylglyoxal and the other (Fr2), rich in polyphenols. Particles were loaded with Fr1, fraction able to enhance in vitro proliferation of human fibroblasts, and with PL. The presence of CS in Fr1-loaded particles produced an improvement in cell proliferation. Moreover, PL loading into particles did not affect the biological activity of the hemoderivative. In vivo efficacy of PL- and Fr1-loaded particles was evaluated on a rat wound model. Both treatments markedly increased wound healing to the same extent. |
| Databáze: | OpenAIRE |
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