Quantitative Analysis of Mycobacterial and Propionibacterial DNA in Lymph Nodes of Japanese and European Patients with Sarcoidosis
| Autor: | Tamiko Takemura, Ikuo Ishige, Touichiro Takizawa, Masayuki Ando, Yoshinobu Eishi, Morio Koike, Ronald M. du Bois, Andrew G. Nicholson, Shoji Kudoh, Daisuke Kobayashi, Gianfranco Rizzato, Moritaka Suga, Ulrich Costabel, Josune Guzman, Om P. Sharma, Tetsuo Yamada, Marcello Gambacorta |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Systemic disease Tuberculosis Sarcoidosis Propionibacterium Paratuberculosis Polymerase Chain Reaction Sensitivity and Specificity Mycobacterium Microbiology Mycobacterium tuberculosis Propionibacterium acnes stomatognathic system Japan medicine Humans Taq Polymerase integumentary system biology Reproducibility of Results Bacteriology biology.organism_classification medicine.disease Europe stomatognathic diseases Immunology Lymph Nodes Actinomycetales Infections |
| Zdroj: | Journal of Clinical Microbiology. 40:198-204 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.1.198-204.2002 |
| Popis: | The cause(s) of sarcoidosis is unknown.Mycobacteriumspp. are suspected in Europe andPropionibacteriumspp. are suspected in Japan. The present international collaboration evaluated the possible etiological links between sarcoidosis and the suspected bacterial species. Formalin-fixed and paraffin-embedded sections of biopsy samples of lymph nodes, one from each of 108 patients with sarcoidosis and 65 patients with tuberculosis, together with 86 control samples, were collected from two institutes in Japan and three institutes in Italy, Germany, and England. Genomes ofPropionibacterium acnes,Propionibacterium granulosum,Mycobacterium tuberculosis,Mycobacterium aviumsubsp.paratuberculosis, andEscherichia coli(as the control) were counted by quantitative real-time PCR. EitherP. acnesorP. granulosumwas found in all but two of the sarcoid samples.M. aviumsubsp.paratuberculosiswas found in no sarcoid sample.M. tuberculosiswas found in 0 to 9% of the sarcoid samples but in 65 to 100% of the tuberculosis samples. In sarcoid lymph nodes, the total numbers of genomes ofP. acnesorP. granulosumwere far more than those ofM. tuberculosis. P. acnesorP. granulosumwas found in 0 to 60% of the tuberculosis and control samples, but the total numbers of genomes ofP. acnesorP. granulosumin such samples were less than those in sarcoid samples.Propionibacteriumspp. are more likely thanMycobacteriaspp. to be involved in the etiology of sarcoidosis, not only in Japanese but also in European patients with sarcoidosis. |
| Databáze: | OpenAIRE |
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