The acute and temporary modulation ofPERIODgenes by hydrocortisone in healthy subjects
| Autor: | Hartmut Schächinger, Thomas M. Schilling, Monika Kölsch, Jobst Meyer, Andrea B. Schote, Türkan Yurtsever, Jonathan D. Turner |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine endocrine system medicine.medical_specialty Hydrocortisone Physiology medicine.medical_treatment Anti-Inflammatory Agents Biology Young Adult 03 medical and health sciences Receptors Glucocorticoid Glucocorticoid receptor Physiology (medical) Internal medicine medicine Humans RNA Messenger Circadian rhythm Whole blood Dose-Response Relationship Drug Period Circadian Proteins PER2 Mifepristone PER3 Steroid hormone 030104 developmental biology Endocrinology Gene Expression Regulation Female hormones hormone substitutes and hormone antagonists Transcription Factors medicine.drug PER1 |
| Zdroj: | Chronobiology International. 33:1222-1234 |
| ISSN: | 1525-6073 0742-0528 |
| DOI: | 10.1080/07420528.2016.1211668 |
| Popis: | The physiological stress system and the circadian clock system communicate with each other at different signaling levels. The steroid hormone cortisol, the end-effector of the hypothalamus-pituitary-adrenal axis, is released in response to stress and acts as a mediator in circadian rhythms. We determined the effect of escalating cortisol doses on the expression of PERIOD genes (PER1, PER2 and PER3) in healthy subjects and analyzed whether the glucocorticoid receptor (GR) is involved in the cortisol-mediated PERIOD gene expression. Forty participants (50% males and 50% females) were randomly assigned to groups receiving a saline placebo solution or 3 mg, 6 mg, 12 mg and 24 mg of hydrocortisone. Blood was drawn every 15 min to measure quantitative gene expression of PER1, PER2 and PER3. A potential role of the GR was determined by an ex vivo study stimulating whole blood with hydrocortisone and RU486 (a GR antagonist). As a result, moderate doses of hydrocortisone produced an acute and temporary induction of PER1 and PER3 mRNA levels, whereas PER2 was not responsive to the hormone administration. The cortisol-dependent induction of PER1 was blocked by the GR antagonist in whole blood after treatment with hydrocortisone and RU486 ex vivo. In conclusion, acute pharmacological stress modulated the expression of PER1 and PER3 in whole blood temporarily in our short-term sampling design, suggesting that these circadian genes mediate stable molecular mechanisms in the periphery. |
| Databáze: | OpenAIRE |
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