Probing the Hydrophobic Cavity of Lipid Transfer Protein from Nicotiana tabacum through Xenon-Based NMR Spectroscopy
| Autor: | Patrick Berthault, Lionel Dubois, Céline Landon, Françoise Vovelle, J. Gaspard Huber, Hervé Desvaux, Pedro Da Silva, Michel Ponchet |
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| Přispěvatelé: | Département de Recherche sur l'Etat Condensé, les Atomes et les Molécules (DRECAM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 'Interactions Plantes-Microorganismes et Santé Végétale' (U.M.R. - I.P.M.S.V.), Institut National de la Recherche Agronomique (INRA), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Rok vydání: | 2004 |
| Předmět: |
Models
Molecular inorganic chemicals Xenon [SDV]Life Sciences [q-bio] Analytical chemistry chemistry.chemical_element 010402 general chemistry 01 natural sciences Biochemistry Catalysis Colloid and Surface Chemistry Chemical affinity Tobacco Isotopes of xenon Molecule Computer Simulation cardiovascular diseases Nuclear Magnetic Resonance Biomolecular Plant Proteins integumentary system 010405 organic chemistry Chemical shift Noble gas General Chemistry Nuclear magnetic resonance spectroscopy Antigens Plant Binding constant 0104 chemical sciences Solutions Kinetics Crystallography chemistry Thermodynamics Carrier Proteins Hydrophobic and Hydrophilic Interactions circulatory and respiratory physiology |
| Zdroj: | Journal of the American Chemical Society Journal of the American Chemical Society, 2004, 126 (48), pp.15738-15746. ⟨10.1021/ja046195i⟩ Scopus-Elsevier Journal of the American Chemical Society, American Chemical Society, 2004, 126 (48), pp.15738-15746. ⟨10.1021/ja046195i S0002-7863(04)06195-5⟩ Journal of the American Chemical Society, American Chemical Society, 2004, 126 (48), pp.15738-15746. ⟨10.1021/ja046195i⟩ |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja046195i |
| Popis: | The hydrophobic cavity of Lipid Transfer Protein 1 from Nicotiana tabacum is investigated in detail by NMR using xenon as a spy. The analysis of the (129)Xe chemical shifts and self-relaxation times gives evidence of protein-xenon interaction. Thermodynamics of the binding is characterized through the study of aliphatic (1)H and (13)C chemical shift variation as a function of xenon pressure. The binding constant is evaluated to 75.5 +/- 1.0 M(-1) at 293 K. The location of xenon inside the cavity is deduced from SPINOE experiments. The noble gas appears to occupy four sites, and xenon self-relaxation experiments indicate that it quickly jumps between different sites. The chemical shifts of amide protons and nitrogens also depend on the xenon concentration, either specifically or nonspecifically for atoms at the external surface of the protein. Yet, contrary to aliphatic atoms, they do not correspond to short-range interactions as confirmed by magnetization transfer experiments between laser-polarized xenon and protons in H(2)O. These (15)N chemical shift variations, used in combination with (15)N transverse self-relaxation rates to determine the lower limit of the binding rate, consequently reveal subtle changes in the structure of the protein upon binding. |
| Databáze: | OpenAIRE |
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