High-performance liquid chromatographic assay of mepivacaine enantiomers in human plasma in the nanogram per milliliter range
| Autor: | J. W. Van Kleef, W. Olieman, K. Groen, A. A. Vletter, Anton G. L. Burm |
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| Rok vydání: | 1996 |
| Předmět: |
Chromatography
Aqueous solution Microchemistry Extraction (chemistry) Stereoisomerism General Chemistry Buffer solution High-performance liquid chromatography Pentane chemistry.chemical_compound chemistry Mepivacaine Humans Regression Analysis Anesthetics Local Diethyl ether Enantiomer Phosphoric acid Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 678:369-372 |
| ISSN: | 0378-4347 |
| Popis: | A method enabling quantification of R-(-)- and S-(+)-mepivacaine in human plasma in the low nanogram per milliliter range is described. The procedure involves extraction from plasma with diethyl ether, centrifugation, back-extraction into an acidified aqueous solution, washing with a mixture of pentane and isoamylalcohol, alkalinisation, followed by extraction with a mixture of n-pentane and isoamylalcohol. After evaporation of the organic phase, the residue is redissolved in the mobile phase used for the HPLC analysis, which consists of a 6.8:93.2 (v/v) isopropanol-sodium hydrogenphosphate buffer solution with the pH adjusted to 6.8 using phosphoric acid. The HPLC method has been described previously. Separation of the enantiomers is achieved with an alpha 1-AGP column and the UV detection wavelength is 210 nm. The minimal detectable concentration is ca. 3 ng/ml and the lower limit of quantification is 5 ng/ml for each enantiomer. For both enantiomers r is > 0.9995 over the plasma enantiomeric concentration range of 10.5-1053 ng/ml. |
| Databáze: | OpenAIRE |
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