Identification and frequency of the associated genes with virulence and antibiotic resistance of Escherichia coli isolated from cow's milk presenting mastitis pathology

Autor: Kateryna Krylova, Hossein Jamali, Mohammed Aider
Přispěvatelé: Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Université Laval [Québec] (ULaval), The technical support of the microbiological laboratory is acknowledged.
Rok vydání: 2018
Předmět:
0301 basic medicine
MESH: Escherichia coli/drug effects
Pathology
antibiotic resistance
cow mastitis
MESH: Milk/microbiology
MESH: Genes
Bacterial
Mastitis
medicine.disease_cause
Integron
MESH: Streptomycin/pharmacology
MESH: Drug Resistance
Bacterial/genetics
MESH: Anti-Bacterial Agents/pharmacology
Genotype
MESH: Animals
MESH: Phylogeny
Phylogeny
MESH: Mastitis/microbiology
biology
Virulence
General Medicine
Antimicrobial
Anti-Bacterial Agents
MESH: Cattle
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Milk
Streptomycin
Female
MESH: Genetic Association Studies
General Agricultural and Biological Sciences
medicine.drug
medicine.medical_specialty
Tetracycline
MESH: Escherichia coli/genetics
MESH: Escherichia coli/isolation & purification
030106 microbiology
03 medical and health sciences
Drug Resistance
Bacterial
medicine
Escherichia coli
Animals
MESH: Virulence/genetics
Genetic Association Studies
MESH: Escherichia coli/pathogenicity
virulence genes
E. coli
medicine.disease
MESH: Tetracycline/pharmacology
Genes
Bacterial
biology.protein
phylogenetic subgroups
Cattle
MESH: Female
Zdroj: Animal Science Journal
Animal Science Journal, Wiley, 2018, 89 (12), pp.1701-1706. ⟨10.1111/asj.13093⟩
ISSN: 1740-0929
1344-3941
Popis: International audience; Antimicrobial resistance, genotype, and virulence gene content of Escherichia coli isolated from bovine clinical mastitis in Tehran Province, Iran, were studied. Two hundred seven (207) milk samples from individual cows presenting mastitis symptoms collected from different dairy farms were used to determine the presence of specific genes of E. coli responsible for this pathology. Multiplex PCR was used to differentiate E. coli isolates into different phylogenetic groups/subgroups and to detect their virulence and involved resistance genes. All the isolated strains were tested for the susceptibility to 21 antimicrobial agents. The results showed that E. coli was detected in 42 (20.3%) samples and 69% of them belonged to the phylogenetic groups A and B1. The phylogenetic subgroup A1 (31%) and subgroup B1 (28.6%) demonstrated the highest prevalence of virulence genes (f17c-A, and eae (n = 6), f17b-A, and f17d-A (n = 5), afaD-8, afaE-8, aucD, and bfpA (n = 4), clpG and VT (n = 2), and LT and ST genes (n = 1)). The highest antimicrobial resistance was observed for tetracycline (45.2%) followed by streptomycin (26.2%). The antimicrobial resistance genes tetB (31%), tetA (28.6%), and aadA (26.2%) were the most prevalent. Moreover, integron class 1 and 2 were found in 24 (57.1%) and 8 (19%) of the E. coli isolates.
Databáze: OpenAIRE
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