Acid-sensing channels in human bladder: expression, function and alterations during bladder pain syndrome
| Autor: | Veronica Sanchez-Freire, Stephan Kellenberger, Maxime G. Blanchard, Fiona C. Burkhard, Katia Monastyrskaya, Thomas M. Kessler |
|---|---|
| Přispěvatelé: | University of Zurich, Monastyrskaya, K |
| Rok vydání: | 2011 |
| Předmět: |
2748 Urology
Male Pathology medicine.medical_specialty Patch-Clamp Techniques Urology Urinary Bladder 030232 urology & nephrology TRPV1 Cystitis Interstitial TRPV Cation Channels 610 Medicine & health Nerve Tissue Proteins urologic and male genital diseases Immunofluorescence Sodium Channels Cell Line Amiloride 03 medical and health sciences 0302 clinical medicine medicine Humans Patch clamp Urothelium Acid-sensing ion channel Ion channel Cells Cultured Aged Urinary bladder medicine.diagnostic_test business.industry Middle Aged Amiloride/pharmacology Capsaicin/pharmacology Cystitis Interstitial/metabolism Cystitis Interstitial/physiopathology Electrophysiological Phenomena/drug effects Female Immunohistochemistry Nerve Tissue Proteins/metabolism Sodium Channels/metabolism TRPV Cation Channels/metabolism Urinary Bladder/metabolism Urothelium/metabolism 3. Good health Electrophysiological Phenomena Acid Sensing Ion Channels medicine.anatomical_structure 10046 Balgrist University Hospital Swiss Spinal Cord Injury Center Capsaicin business 030217 neurology & neurosurgery |
| Zdroj: | The Journal of urology Journal of Urology, vol. 186, no. 4, pp. 1509-1516 |
| DOI: | 10.1016/j.juro.2011.05.047 |
| Popis: | PURPOSE We examined the possible role of H(+) activated acid sensing ion channels in pain perception. We characterized expression in bladder dome biopsies from patients with bladder pain syndrome and controls in cultured human urothelium and in urothelial TEU 2 cells. MATERIALS AND METHODS Cold cut biopsies from the bladder dome were obtained in 8 asymptomatic controls and 28 patients with bladder pain syndrome symptoms. Acid sensing ion channel expression was analyzed by quantitative real time polymerase chain reaction and immunofluorescence. Channel function was measured by electrophysiology. RESULTS Acid sensing ion channel 1a 2a and 3 mRNA was detected in the human bladder. Similar amounts of acid sensing ion channel 1a and 3 were detected in detrusor smooth muscle while in urothelium acid sensing ion channel 3 levels were higher than levels of acid sensing ion channel 1a. Acid sensing ion channel 2a mRNA levels were lower than acid sensing ion channel 1a and 3 levels in each layer. Acid sensing ion channel currents were measured in TEU 2 cells and in primary cultures of human urothelium. Activated acid sensing ion channel expression was confirmed by quantitative real time polymerase chain reaction. TEU 2 cell differentiation caused acid sensing ion channel 2a and 3 mRNA up regulation and acid sensing ion channel 1a mRNA down regulation. Patients with bladder pain syndrome showed up regulation of acid sensing ion channel 2a and 3 mRNA but acid sensing ion channel 1a remained unchanged. In contrast transient receptor potential vanilloid 1 mRNA was down regulated during bladder pain syndrome. All differences were statistically significant (p |
| Databáze: | OpenAIRE |
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