Neuroanatomical Distribution of DEK Protein in Corticolimbic Circuits Associated with Learning and Memory in Adult Male and Female Mice
| Autor: | Christina M. Estrada, Matia B. Solomon, Valentina Ghisays, Joshua Streicher, Ana Franco-Villanueva, Nicholas A. Pease, Elizabeth T. Nguyen, Lisa M. Privette Vinnedge, Maureen Fitzgerald |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Central nervous system Hippocampus Article 03 medical and health sciences Memory Neural Pathways Limbic System medicine Animals Learning Poly-ADP-Ribose Binding Proteins Prefrontal cortex Cerebral Cortex Mice Knockout Neurons Oncogene Proteins Oncogene General Neuroscience Dentate gyrus Wnt signaling pathway Subiculum Entorhinal cortex Immunohistochemistry DNA-Binding Proteins Mice Inbred C57BL stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Astrocytes Female Microglia Psychology Neuroscience |
| Zdroj: | Neuroscience. 371:254-267 |
| ISSN: | 0306-4522 |
| Popis: | DEK, a chromatin-remodeling gene expressed in most human tissues, is known for its role in cancer biology and autoimmune diseases. DEK depletion in vitro reduces cellular proliferation, induces DNA damage subsequently leading to apoptosis, and down-regulates canonical Wnt/β-catenin signaling, a molecular pathway essential for learning and memory. Despite a recognized role in cancer (non-neuronal) cells, DEK expression and function is not well characterized in the central nervous system. We conducted a gene ontology analysis (ToppGene), using a cancer database to identify genes associated with DEK deficiency, which pinpointed several genes associated with cognitive-related diseases (i.e., Alzheimer’s disease, presenile dementia). Based on this information, we examined DEK expression in corticolimbic structures associated with learning and memory in adult male and female mice using immunohistochemistry. DEK was expressed throughout the brain in both sexes, including the medial prefrontal cortex (prelimbic, infralimbic and dorsal peduncular). DEK was also abundant in all amygdalar subdivisions (basolateral, central and medial) and in the hippocampus including the CA1, CA2, CA3, dentate gyrus (DG), ventral subiculum and entorhinal cortex. Of note, compared to males, females had significantly higher DEK immunoreactivity in the CA1, indicating a sex difference in this region. DEK was co-expressed with neuronal and microglial markers in the CA1 and DG, whereas only a small percentage of DEK cells were in apposition to astrocytes in these areas. Given the reported inverse cellular and molecular profiles (e.g., cell survival, Wnt pathway) between cancer and Alzheimer’s disease, these findings suggest a potentially important role of DEK in cognition. |
| Databáze: | OpenAIRE |
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