Inhalation of high-concentration hydrogen gas attenuates cognitive deficits in a rat model of asphyxia induced-cardiac arrest
| Autor: | Warren Boling, Patricia M Applegate, John H. Zhang, Lei Huang, Umut Ocak, Richard Lee Applegate, Lei Gong |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Neuroscience (miscellaneous) Morris water navigation task Water maze cardiac arrest 030204 cardiovascular system & hematology Hippocampal formation cognitive deficit Rats Sprague-Dawley 03 medical and health sciences Asphyxia 0302 clinical medicine Cognition Administration Inhalation medicine water maze Animals rat high concentration hydrogen gas Maze Learning Cognitive deficit Inhalation Dose-Response Relationship Drug business.industry Brain brain resuscitation neuron Heart Arrest Rats Disease Models Animal Anesthesiology and Pain Medicine medicine.anatomical_structure global brain ischemia Anesthesia Neuron medicine.symptom business 030217 neurology & neurosurgery Ex vivo Research Article Hydrogen |
| Zdroj: | Medical Gas Research Medical Gas Research, vol 9, iss 3 |
| ISSN: | 2045-9912 |
| Popis: | Cognitive deficits are a devastating neurological outcome seen in survivors of cardiac arrest. We previously reported water electrolysis derived 67% hydrogen gas inhalation has some beneficial effects on short-term outcomes in a rat model of global brain hypoxia-ischemia induced by asphyxia cardiac arrest. In the present study, we further investigated its protective effects in long-term spatial learning memory function using the same animal model. Water electrolysis derived 67% hydrogen gas was either administered 1 hour prior to cardiac arrest for 1 hour and at 1-hour post-resuscitation for 1 hour (pre- & post-treatment) or at 1-hour post-resuscitation for 2 hours (post-treatment). T-maze and Morris water maze were used for hippocampal memory function evaluation at 7 and 14 days post-resuscitation, respectively. Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo. Hippocampal deficits were detected at 7 and 18 days post-resuscitation, with increased neuronal degeneration within hippocampal CA1 regions. Both hydrogen gas treatment regimens significantly improved spatial learning function and attenuated neuronal degeneration within hippocampal CA1 regions at 18 days post-resuscitation. Our findings suggest that water electrolysis derived 67% hydrogen gas may be an effective therapeutic approach for improving cognitive outcomes associated with global brain hypoxia-ischemia following cardiac arrest. The study was approved by the Animal Health and Safety Committees of Loma Linda University, USA (approval number: IACUC #8170006) on March 2, 2017. |
| Databáze: | OpenAIRE |
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