Combined 1,25-Dihydroxy-vitamin D and Resveratrol: A Novel Therapeutic Approach to Ameliorate Ischemia Reperfusion-Induced Myocardial Injury
| Autor: | Asefeh Fekri, Shahnaz Shekarforoush, Seyedhossein Hekmatimoghaddam, Fatemeh Safari, Mohsen Sharifi Klishadi, Farideh Zarei |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Myocardial Infarction Ischemia Medicine (miscellaneous) chemistry.chemical_element Resveratrol Calcium Real-Time Polymerase Chain Reaction chemistry.chemical_compound Internal medicine Stilbenes Vitamin D and neurology Animals Medicine RNA Messenger Myocardial infarction Rats Wistar Vitamin D Nutrition and Dietetics biology business.industry Anti-Inflammatory Agents Non-Steroidal Arrhythmias Cardiac Vitamins General Medicine medicine.disease Rats Endocrinology chemistry Biochemistry Catalase Reperfusion Injury biology.protein Drug Therapy Combination Ligation business Reperfusion injury |
| Zdroj: | International Journal for Vitamin and Nutrition Research. 85:174-184 |
| ISSN: | 1664-2821 0300-9831 |
| DOI: | 10.1024/0300-9831/a000236 |
| Popis: | Abstract. The aim of this study was to assess the effect of combined 1,25-dihydroxyvitamin D (1,25 D) and resveratrol on cardiac arrhythmias, infarct size, and transcription of catalase, thioredoxin-1 and B-cell lymphoma 2 (Bcl-2), following myocardial ischemia-reperfusion (IR) in male rats. Ligation of coronary artery was performed in rats (n = 6 per group) without any treatment (IR group), pretreated with 0.1 μg/kg/day of 1,25 D (1,25 D + IR), 1 mg/kg/day of resveratrol (Res + IR) or a combination (1,25 D + Res + IR) for 14 days. Arrhythmias were analyzed according to the Lambeth conventions, and infarct size was measured by 2,3,5-triphenyl-2H-tetrazolium chloride staining. Expression of prosurvival genes was evaluated by real-time polymerase chain reaction. In the 1,25 D + Res + IR group the mean infarct size was 17.6 ± 3.5 %, which was significantly less than that in the IR, 1,25 D + IR, and Res + IR groups (p < 0.001). Although the single therapy of either 1,25 D or resveratrol did not change the incidence of arrhythmias significantly, a reduction in the number of ventricular ectopic beats was noted in group 1,25 D + Res + IR (179.19 ± 58.87, p < 0.001 vs IR; p < 0.05 vs Res + IR; p < 0.01 vs Vit D + IR). Combination of 1,25 D and resveratrol increased transcription of catalase by 119 ± 37 % (p < 0.001 vs IR, p < 0.01 vs Res + IR, p < 0.001 vs 1,25 D + IR). Our study showed that combination of a non-hypotensive dose of 1,25 D and resveratrol can be a novel and effective strategy for protecting against ischemia. |
| Databáze: | OpenAIRE |
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