| Autor: |
Huber, SM, van Delft, P, Tanpure, A, Miska, EA, Balasubramanian, S |
| Přispěvatelé: |
van Delft, Pieter [0000-0002-4726-9467], Tanpure, Arun [0000-0002-5227-8376], Miska, Eric [0000-0002-4450-576X], Balasubramanian, Shankar [0000-0002-0281-5815], Apollo - University of Cambridge Repository |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| DOI: |
10.17863/cam.8827 |
| Popis: |
5-Hydroxymethylcytidine (hm$^{5}$C) was recently identified as a direct metabolite of m$^{5}$C in RNA. We investigated the stability of hm$^{5}$C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m$^{5}$C in RNA, namely 2′-$\textit{0}$-methyl-5-hydroxymethylcytidine (hm$^{5}$C$\textit{m}$). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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