Isolation of mutant T lymphocytes with defects in capacitative calcium entry
| Autor: | Leonard A. Herzenberg, Christopher M. Fanger, Andrew T. Serafini, Gerald R. Crabtree, Richard S. Lewis, Neil A. Clipstone, Steve Flering, Richard J. Bram |
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| Rok vydání: | 1995 |
| Předmět: |
T-Lymphocytes
T cell Molecular Sequence Data Immunology Biology Lymphocyte Activation Jurkat cells Cell Line Transcription (biology) Phosphoprotein Phosphatases medicine Humans Immunology and Allergy Cytotoxic T cell computer.programming_language Cell Nucleus Regulation of gene expression Base Sequence NFATC Transcription Factors Caml Calcineurin Nuclear Proteins Molecular biology Cell Compartmentation Cell biology DNA-Binding Proteins Infectious Diseases medicine.anatomical_structure Gene Expression Regulation Oligodeoxyribonucleotides Cell culture Mutation Calcium Calmodulin-Binding Proteins computer Transcription Factors |
| Zdroj: | Immunity. 3:239-250 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/1074-7613(95)90093-4 |
| Popis: | Calcium and calcium-binding proteins play important roles in the signaling cascade leading from the initial engagement of TCRs on T cells to the fully activated state. To undertake a molecular dissection of this cascade, we first isolated a Jurkat T cell line derivative containing the NF-AT promoter element driving transcription of the diphtheria toxin A chain gene ( dipA ), resulting in rapid cell death. Selecting viable cells that fail to activate NF-AT-dependent transcription, we isolated two independent cell lines possessing defects in capacitative Ca 2+ entry. NF-AT-dependent transcription can be restored in these cells by expression of a constitutively active calcineurin, but not by overexpression of the Ca 2+ regulatory protein CAML, which can normally replace the Ca 2+ signal. The defect in these cell lines probably lies between CAML and calcineurin in the T cell activation cascade. |
| Databáze: | OpenAIRE |
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