Bordetella pertussis iron regulated proteins as potential vaccine components
| Autor: | Esteban Erben, Jimena Alvarez Hayes, Fabricio Alejandro Maschi, Yanina Andrea Lamberti, Miguel Ángel Ayala, Guido Mariano Principi, María Eugenia Rodríguez |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Bordetella pertussis
CIENCIAS MÉDICAS Y DE LA SALUD Neutrophils Whooping Cough Ciencias de la Salud Microbiology Serology law.invention Mice Antigen law medicine Animals Humans Iron Regulatory Protein 1 Cells Cultured Whooping cough Pertussis Vaccine Antigens Bacterial Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology New antigens Vaccination Public Health Environmental and Occupational Health Opsonin Proteins biology.organism_classification medicine.disease Antibodies Bacterial Virology Enfermedades Infecciosas Infectious Diseases Immunization Proteome Recombinant DNA biology.protein Molecular Medicine Female Antibody Vaccine Bacterial Outer Membrane Proteins |
| Zdroj: | Vaccine. 31:3543-3548 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2013.05.072 |
| Popis: | Bordetella pertussis is the etiologic agent of whooping cough, an illness whose incidence has been increasing over the last decades. Pertussis reemergence despite high vaccination coverage, together with the recent isolation of circulating strains deficient in some of the vaccine antigens, highlight the need for new vaccines. Proteins induced under physiological conditions, such as those required for nutrient acquisition during infection, might represent good targets for better preventive strategies. By mean of serological proteome analysis we identified two novel antigens of B. pertussis potentially involved in iron acquisition during host colonization. We had previously demonstrated that one of them, designated IRP1-3, is protective against pertussis infection in mice. In the present study, we show that the other antigen, named AfuA (BP1605), is a highly antigenic protein, exposed on the bacterial surface, conserved among clinical isolates and expressed during infection. Immunization of mice with the recombinant AfuA induced opsonophagocytic antibodies which could explain the protection against B. pertussis infection conferred by mice immunization with rAfuA. Importantly, we found that the addition of rAfuA and rIRP1-3 proteins to the commercial three pertussis components acellular vaccine significantly increased its protective activity. Taken together, our results point at these two antigens as potential components of a new generation of acellular vaccines Fil: Alvarez Hayes, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina Fil: Erben, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina Fil: Lamberti, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina Fil: Principi, Guido. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Animales de Laboratorio; Argentina Fil: Maschi, Fabricio. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Animales de Laboratorio; Argentina Fil: Ayala, Miguel Angel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Animales de Laboratorio; Argentina Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina |
| Databáze: | OpenAIRE |
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