Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs
| Autor: | William Sosna, Micah D. Halpern, Sara B Hamilton, Julie M Owells, Deirdre E Daniels, Eric R Jeppesen, Kimberly S. McCurdy, John A. Lednicky, Jon O. Rayner |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Virus Cultivation
Biosafety cabinet Chick Embryo Biology medicine.disease_cause Virus Cell Line Microbiology lcsh:Infectious and parasitic diseases Occupational Exposure Biosafety level Virology Chlorocebus aethiops medicine Animals Humans lcsh:RC109-216 Microbial Viability Inoculation Ectromelia virus Methodology Embryonated Containment of Biohazards Ethylenes biology.organism_classification Survival Analysis Influenza A virus subtype H5N1 Infectious Diseases Viruses Disinfectants |
| Zdroj: | Virology Journal, Vol 7, Iss 1, p 23 (2010) Virology Journal |
| Popis: | BackgroundEmbryonated chicken eggs (ECE) are sometimes used for the primary isolation or passage of influenza viruses, other viruses, and certain bacteria. For small-scale experiments with pathogens that must be studied in biosafety level three (BSL3) facilities, inoculated ECE are sometimes manipulated and maintained in small egg incubators within a biosafety cabinet (BSC). To simplify the clean up and decontamination of an egg incubator in case of egg breakage, we explored whether ethylene breather bags could be used to encase ECE inoculated with pathogens. This concept was tested by determining embryo survival and examining virus yields in bagged ECE.ResultsVirus yields acceptable for many applications were attained when influenza-, alpha-, flavi-, canine distemper-, and mousepox viruses were propagated in ECE sealed within ethylene breather bags.ConclusionsFor many small-scale applications, ethylene breather bags can be used to encase ECE inoculated with various viruses. |
| Databáze: | OpenAIRE |
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