Blood CD34−c-Kit+ cell rate correlates with aggressive forms of systemic mastocytosis and behaves like a mast cell precursor
| Autor: | Patrice Dubreuil, Stéphane Barete, Olivier Lortholary, Fanny Lanternier, Mohamed Hamidou, Yves Lepelletier, Frédéric Féger, Sophie Georgin-Lavialle, Olivier Hermine, Cédric Baude, Marie-Olivia Chandesris, Gandhi Damaj, Katia Hanssens, Julie Bruneau, Christian de Gennes, Jean-Marie Launay, Ludovic Lhermitte |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Immunology Cell Population CD34 Antigens CD34 Biochemistry Flow cytometry Mastocytosis Systemic medicine Humans Mast Cells Prospective Studies Systemic mastocytosis Child education Aged education.field_of_study Blood Cells medicine.diagnostic_test business.industry Stem Cells Case-control study Cell Biology Hematology Middle Aged Flow Cytometry medicine.disease Mast cell Proto-Oncogene Proteins c-kit medicine.anatomical_structure Case-Control Studies Mutation Female Stem cell business |
| Zdroj: | Blood. 118:5246-5249 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2011-02-335950 |
| Popis: | Mastocytosis is a heterogeneous disease characterized by the accumulation of mast cells in one or more organs. Our objective was to identify a peripheral mast cell precursor and assess its variation rate in mastocytosis. A peripheral blood phenotypic analysis was performed among 50 patients with mastocytosis who were enrolled in a prospective multicentric French study, and the phenotypic analysis results of the patients were compared with those of healthy donors. The rate of peripheral blood CD34−c-Kit+ cells correlated with the severity of mastocytosis. This cellular population was isolated from healthy donors as well as from patients with systemic mastocytosis. After 30 days of culture, the CD34−c-Kit+ cells gave birth to mature mast cells, indicating that this cellular population constitutes a mast cell circulating precursor. Monitoring peripheral CD34−c-Kit+ cells by flow cytometry could be a useful and low-invasive tool to determine the disease severity and the relapses and to assess treatment efficiency. |
| Databáze: | OpenAIRE |
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