Mono-2-ethylhexyl phthalate induces the expression of genes involved in fatty acid synthesis in HepG2 cells
| Autor: | Qing Tan, Zhen He, Jianying Bai, Hongmei Yu, Yaofu Li, Xuexia Jiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Health Toxicology and Mutagenesis 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Diethylhexyl Phthalate Internal medicine medicine Humans Carbohydrate-responsive element-binding protein Gene Triglycerides Fatty acid synthesis 030304 developmental biology 0105 earth and related environmental sciences Pharmacology chemistry.chemical_classification 0303 health sciences Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Fatty liver Phthalate Fatty acid Hep G2 Cells General Medicine Lipid Metabolism medicine.disease Fatty Acid Synthase Type I Endocrinology chemistry Hepg2 cells Sterol Regulatory Element Binding Protein 1 Stearoyl-CoA Desaturase Intracellular Acetyl-CoA Carboxylase |
| Zdroj: | Environmental Toxicology and Pharmacology. 69:104-111 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/j.etap.2019.04.004 |
| Popis: | Mono-2-ethylhexyl phthalate (MEHP) is a major bioactive metabolite in the widely used industrial plasticizer diethylhexyl phthalate (DEHP) that has been found to be toxic to the liver. The aim of this study is to determine whether MEHP exposure can change the expression of fatty acid metabolism-related genes in HepG2 cells, which might be related to non-alcoholic fatty liver disease (NAFLD). The results revealed that exposure to MEHP promoted lipid accumulation in HepG2 cells. The levels of intracellular triglycerides in the hepatocytes increased after exposure to 0.8–100 μM MEHP for 24 h and 48 h. The genetic expressions of SREBP-1c, ChREBP, ACC1, FASN, and SCD significantly increased at 6 h after exposure to MEHP. At 24 h, the expression of the SREBP-1c and ChREBP genes remained increased, while the expression of the FASN and SCD genes decreased. At 48 h, the expression of SREBP-1c, ChREBP, ACC1, FASN, and SCD decreased. Furthermore, the levels of proteins including ACC1, FASN, SCD, and ChREBP (except SREBP-1c) increased at 24 h. These findings suggest that MEHP exposure can promote fatty acid synthesis in hepatocytes by regulating the expression of relevant genes and proteins, contributing to NAFLD. |
| Databáze: | OpenAIRE |
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