The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells

Autor: Eva Syková, Pavel Rossner, Bozena Novotna, Karolina Turnovcova, Yana Bagryantseva, Pavla Jendelova, Vít Herynek, Mariana Klementová, Magda Vosmanská, Pavel Veverka, Pavel Zvatora
Rok vydání: 2015
Předmět:
Materials science
Cell Survival
Surface Properties
DNA damage
Cell Culture Techniques
Biomedical Engineering
Contrast Media
Nanotechnology
02 engineering and technology
Isoprostanes
Dinoprost
010402 general chemistry
Toxicology
medicine.disease_cause
Ferric Compounds
01 natural sciences
Protein Carbonylation
chemistry.chemical_compound
Microscopy
Electron
Transmission
medicine
Animals
Propidium iodide
Cytotoxicity
Cells
Cultured
Cell Proliferation
Dose-Response Relationship
Drug
Staining and Labeling
Cell growth
Mesenchymal stem cell
Mesenchymal Stem Cells
Cobalt
Lipid Metabolism
Silicon Dioxide
021001 nanoscience & nanotechnology
Magnetic Resonance Imaging
Rats
0104 chemical sciences
Comet assay
Zinc ferrite
chemistry
Zinc Compounds
Biophysics
Nanoparticles
0210 nano-technology
Genotoxicity
DNA Damage
Zdroj: Nanotoxicology. 10:662-670
ISSN: 1743-5404
1743-5390
DOI: 10.3109/17435390.2015.1107144
Popis: Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F2t-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
Databáze: OpenAIRE
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