The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene
| Autor: | Hideya Fukuzawa, Tsutomu Hanajiri, Takefumi Sone, Axel Brennicke, Katsuyuki T. Yamato, Kanji Ohyama, Masaki Fujisawa, Mizuki Takenaka, Kaoru Kono, Yuu Shimizu-Ueda, Kimitsune Ishizaki, Sachiko Okada, Shigeki Nakayama |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
DNA
Plant Molecular Sequence Data Biology Y chromosome Polymerase Chain Reaction Marchantia polymorpha Magnoliopsida Open Reading Frames Chromosome 16 Y Chromosome Gene family Chromosomes Artificial Amino Acid Sequence X chromosome In Situ Hybridization Fluorescence Genetics Multidisciplinary Base Sequence Sequence Homology Amino Acid Chromosome Mapping Biological Sciences biology.organism_classification Molecular biology Chromosome 3 Chromosome 21 Chromosome 22 |
| Popis: | The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of ≈70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2–3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human. |
| Databáze: | OpenAIRE |
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