Effect of alpha-toxin and capsular exopolysaccharide on the adherence of Staphylococcus aureus to cultured teat, ductal and secretory mammary epithelial cells
| Autor: | E Cifrian, C.N O'Brien, W.W. Marquardt, AJ Guidry |
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| Rok vydání: | 1995 |
| Předmět: |
Staphylococcus aureus
General Veterinary Polysaccharides Bacterial Capsule Biology medicine.disease_cause Bacterial Adhesion Epithelium Microbiology Mammary Glands Animal medicine.anatomical_structure Mammary Epithelium Alpha-toxin Type C Phospholipases medicine Animals Cattle Female Collagen Cells Cultured |
| Zdroj: | Research in Veterinary Science. 58:20-25 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(95)90083-7 |
| Popis: | Cultures of teat, ductal and secretory epithelial cells were used to study the role of alpha-toxin and the capsular exopolysaccharide on the adherence of Staphylococcus aureus to mammary epithelium. The adherence of S aureus to the cells and their susceptibility to damage by alpha-toxin increased from teat to ductal to secretory cells. Alpha-toxin increased the susceptibility of epithelial cell monolayers to adherence by S aureus, and the extent of the adherence increased with the time of exposure to alpha-toxin. The exopolysaccharide capsule deterred the adherence of S aureus to mammary epithelial cells and to collagen. Organisms with a rigid capsule adhered to a smaller extent than those with a flaccid capsule. Both encapsulated and unencapsulated S aureus adhered more readily to collagen than to either healthy monolayers of epithelial cells or monolayers of cells damaged by alpha-toxin. |
| Databáze: | OpenAIRE |
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