Fluorescence-guided surgery of a highly-metastatic variant of human triple-negative breast cancer targeted with a cancer-specific GFP adenovirus prevents recurrence
| Autor: | Shuya Yano, Michael Bouvet, Kiyoto Takehara, Shinji Miwa, Robert M. Hoffman, Shunsuke Kagawa, Yasuo Urata, Hiroshi Tazawa, Toshiyoshi Fujiwara, Hiroyuki Kishimoto |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Telomerase Gene Expression Triple Negative Breast Neoplasms Complete resection Metastasis Green fluorescent protein Mice Computer-Assisted 0302 clinical medicine Recurrence Transduction Genetic Triple-negative breast cancer Cells Cultured Microscopy Cultured Tumor Optical Imaging adenovirus nude mice 3. Good health Oncology Surgery Computer-Assisted 030220 oncology & carcinogenesis Female fluorescence-guided surgery Research Paper medicine.medical_specialty Cells Oncology and Carcinogenesis Genetic Vectors Green Fluorescent Proteins survival Fluorescence triple negative Cell Line Adenoviridae Transduction 03 medical and health sciences breast cancer Breast cancer Genetic Cell Line Tumor medicine Animals Humans orthotopic Animal business.industry Cancer GFP/RFP medicine.disease Xenograft Model Antitumor Assays Surgery Disease Models Animal 030104 developmental biology Microscopy Fluorescence Disease Models Cancer cell business fluorescence-guided surgery (FGS) telomerase dependent |
| Zdroj: | Oncotarget Oncotarget, vol 7, iss 46 |
| ISSN: | 1949-2553 |
| Popis: | // Shuya Yano 1, 2, 3 , Kiyoto Takehara 1, 2, 3 , Shinji Miwa 1, 2 , Hiroyuki Kishimoto 3 , Hiroshi Tazawa 4 , Yasuo Urata 5 , Shunsuke Kagawa 3 , Michael Bouvet 2 , Toshiyoshi Fujiwara 3 , Robert M. Hoffman 1, 2 1 AntiCancer, Inc., San Diego, CA, USA 2 Department of Surgery, University of California San Diego, CA, USA 3 Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan 4 Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan 5 Oncolys BioPharm Inc., Tokyo, Japan Correspondence to: Robert M. Hoffman, email: all@anticancer.com Toshiyoshi Fujiwara, email: toshi_ f@md.okayama-u.ac.jp Keywords: fluorescence-guided surgery (FGS), telomerase dependent, adenovirus, GFP/RFP, survival Received: July 07, 2016 Accepted: September 06, 2016 Published: September 28, 2016 ABSTRACT We have previously developed a genetically-engineered GFP-expressing telomerase-dependent adenovirus, OBP-401, which can selectively illuminate cancer cells. In the present report, we demonstrate that targeting a triple-negative high-invasive human breast cancer, orthotopically-growing in nude mice, with OBP-401 enables curative fluorescence-guided surgery (FGS). OBP-401 enabled complete resection and prevented local recurrence and greatly inhibited lymph-node metastasis due to the ability of the virus to selectively label and subsequently kill cancer cells. In contrast, residual breast cancer cells become more aggressive after bright (white)-light surgery (BLS). OBP-401-based FGS also improved the overall survival compared with conventional BLS. Thus, metastasis from a highly-aggressive triple-negative breast cancer can be prevented by FGS in a clinically-relevant mouse model. |
| Databáze: | OpenAIRE |
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