Molecular and biochemical analysis of rainbow trout LCK suggests a conserved mechanism for T-cell signaling in gnathostomes
| Autor: | Stacey B. B. Dutton, J. D. Hansen, Kerry J. Laing |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
endocrine system
animal structures T-Lymphocytes animal diseases T cell Molecular Sequence Data Immunology digestive system medicine Animals Tissue Distribution Amino Acid Sequence Molecular Biology Gene Phylogeny Genetics Messenger RNA biology urogenital system Antibodies Monoclonal T lymphocyte biology.organism_classification Recombinant Proteins Cell biology Trout Thymocyte medicine.anatomical_structure Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Oncorhynchus mykiss Rainbow trout Signal transduction Signal Transduction |
| Zdroj: | Molecular Immunology. 44:2737-2748 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2006.11.021 |
| Popis: | Two genes were identified in rainbow trout that display high sequence identity to vertebrate Lck. Both of the trout Lck transcripts are associated with lymphoid tissues and were found to be highly expressed in IgM-negative lymphocytes. In vitro analysis of trout lymphocytes indicates that trout Lck mRNA is up-regulated by T-cell mitogens, supporting an evolutionarily conserved function for Lck in the signaling pathways of T-lymphocytes. Here, we describe the generation and characterization of a specific monoclonal antibody raised against the N-terminal domains of recombinant trout Lck that can recognize Lck protein(s) from trout thymocyte lysates that are similar in size ( approximately 57kDa) to mammalian Lck. This antibody also reacted with permeabilized lymphocytes during FACS analysis, indicating its potential usage for cellular analyses of trout lymphocytes, thus representing an important tool for investigations of salmonid T-cell function. |
| Databáze: | OpenAIRE |
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