Assessment of resistomycin, as an anticancer compound isolated and characterized from Streptomyces aurantiacus AAA5
| Autor: | Venkatesan Brindha Priyadarisini, Duraisamy Senthil Raja, Per Bruheim, Sathyaseelan Sathyabama, Rajendran Vijayabharathi, Palanisamy Bruntha Devi, Trygve Andreassen |
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| Rok vydání: | 2011 |
| Předmět: |
Molecular Sequence Data
Antineoplastic Agents Applied Microbiology and Biotechnology Microbiology HeLa chemistry.chemical_compound Pigment Cell Line Tumor Humans Benzopyrenes Phylogeny Soil Microbiology Cell Proliferation Bacteria Streptomyces aurantiacus biology Strain (chemistry) General Medicine biology.organism_classification 16S ribosomal RNA Streptomyces Anti-Bacterial Agents chemistry Biochemistry visual_art visual_art.visual_art_medium Growth inhibition Antibacterial activity |
| Zdroj: | The Journal of Microbiology. 49:920-926 |
| ISSN: | 1976-3794 1225-8873 |
| DOI: | 10.1007/s12275-011-1260-5 |
| Popis: | A new actinomycete strain, isolated from humus soils in the Western Ghats, was found to be an efficient pigment producer. The strain, designated AAA5, was identified as a putative Streptomyces aurantiacus strain based on cultural properties, morphology, carbon source utilization, and analysis of the 16S rRNA gene. The strain produced a reddish-brown pigmented compound during the secondary metabolites phase. A yellow compound was derived from the extracted pigment and was identified as the quinone-related antibiotic resistomycin based on ultraviolet-visible spectrophotometry, fourier transform infrared spectroscopy, liquid chromatography and mass spectroscopy, and nuclear magnetic resonance analyses. The AAA5 strain was found to produce large quantities of resistomycin (52.5 mg/L). It showed potent cytotoxic activity against cell lines viz. HepG2 (hepatic carcinoma) and HeLa (cervical carcinoma) in vitro, with growth inhibition (GI(50)) of 0.006 and 0.005 μg/ml, respectively. The strain also exhibited broad antimicrobial activities against both Gram-positive and Gram-negative bacteria. Therefore, AAA5 may have great potential as an industrial resistomycin-producing strain. |
| Databáze: | OpenAIRE |
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