Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment
| Autor: | Kohei Okubo, Yuki Hiruta, Hideko Kanazawa, Michiko Akimaru |
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| Rok vydání: | 2015 |
| Předmět: |
Surface Properties
Acrylic Resins Analytical Chemistry chemistry.chemical_compound Column chromatography Theophylline medicine Humans Solid phase extraction Serum Albumin Aqueous solution Chromatography Solid Phase Extraction Extraction (chemistry) Temperature Cationic polymerization Analytic Sample Preparation Methods Hydrogels Silicon Dioxide Human serum albumin chemistry Phenytoin Self-healing hydrogels Poly(N-isopropylacrylamide) Hydrophobic and Hydrophilic Interactions medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1348-2246 0910-6340 |
| Popis: | We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogel-modified stationary phase. Aminopropyl silica beads (average diameter, 40 - 64 μm) were coated with poly(N-isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacrylate (BMA) and N,N-dimethylaminopropyl acrylamide (DMAPAAm) were used as the hydrophobic and cationic monomers, respectively, and copolymerized with NIPAAm. To evaluate the use of this SPE cartridge for the analysis of drugs and proteins in biological fluids, we studied the separation of phenytoin and theophylline from human serum albumin (HSA) as a model system. The retention of the analytes in an exclusively aqueous eluent could be modulated by changing the temperature and salt content. These results indicated that this temperature-responsive SPE system can be applied to the pretreatment of biological samples for the measurement of serum drug levels. |
| Databáze: | OpenAIRE |
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