Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
| Autor: | Mike Drebot, Emelissa Mendoza, Annie Mak, Min-Kuang Lee, Jonathan Laley, Martin Petric, Navdeep Chahil, Inna Sekirov, Heidi Wood, Mel Krajden, David Lawrence, Erin Carruthers, Tamara Pidduck, Paul N. Levett, Muhammad Morshed, Jesse Kustra |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
biology
business.industry Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses fungi PRNT Short Communications virus diseases SARS CoV-2 COVID-19 serology neutralization Virology enzyme immunoassay Neutralization Virus body regions Performance comparison biology.protein Medicine General Materials Science Antibody business skin and connective tissue diseases microneutralization |
| Zdroj: | Access Microbiology |
| ISSN: | 2516-8290 |
| Popis: | We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies’ neutralizing capacity outside CL-3 laboratory containment. |
| Databáze: | OpenAIRE |
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