Origin of tryptophan in fructose 1,6-bisphosphatase purified from livers of fed rabbits

Autor:	Edon Melloni, Franca Salamino, Sandro Pontremoli, Charles E. Isaacs, Hamza A. El-Dorry, Mauro Michetti, B.L. Horecker, Douglas K. Chu, Lynne H. Botelho
Rok vydání:	1978
Předmět:	chemistry.chemical_classification Hot Temperature Chromatography biology Aldolase A Tryptophan Biophysics Fructose 1 6-bisphosphatase Fructose Biochemistry Fructose-Bisphosphatase chemistry.chemical_compound Enzyme Liver chemistry biology.protein Animals Rabbits Molecular Biology
Zdroj:	Archives of Biochemistry and Biophysics. 191:825-827
ISSN:	0003-9861
DOI:	10.1016/0003-9861(78)90426-5
Popis:	Fructose 1,6-bisphosphatase (Fru-P2ase,EC 3.1.3.11) purified from livers of fed rabbits has been reported to contain tryptophan, which is not present in the enzyme purified from livers of fasted animals. We now show that the tryptophan arises from small amounts of active or inactive rabbit liver aldolase in the Fru-P2ase preparations. Fru-P2ase free of tryptophan may conveniently be prepared by raising the temperature of the heat step in the purification procedure to 67 °C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7eb4b291e78a875ca95b970892d68906 https://doi.org/10.1016/0003-9861(78)90426-5 Zobrazit plný text záznamu