Conserved TcR β chain usage for a single MHC class II-restricted heat shock protein peptide
Autor: | Linda Struyk, Judy Henwood, P.J. van den Elsen, Gail E. Hawes, J. S. Hill Gaston, Helen Beacock-Sharp |
---|---|
Rok vydání: | 1998 |
Předmět: |
Chaperonins
Receptors Antigen T-Cell alpha-beta T-Lymphocytes Molecular Sequence Data Immunology Clone (cell biology) Tuberculoid leprosy Peptide Biology Immunoglobulin alpha-Chains HLA-DR3 Antigen Bacterial Proteins Antigen Heat shock protein Genetics medicine Humans Amino Acid Sequence Gene Rearrangement beta-Chain T-Cell Antigen Receptor Gene chemistry.chemical_classification Antigen Presentation MHC class II Base Sequence Sequence Homology Amino Acid T-cell receptor Chaperonin 60 DNA medicine.disease Complementarity Determining Regions Leprosy Tuberculoid Clone Cells chemistry biology.protein |
Zdroj: | Immunogenetics. 48:196-201 |
ISSN: | 1432-1211 0093-7711 |
Popis: | Previously we have shown that the T-cell response against the HLA-DR3 (17)-restricted heat shock protein (Mr 65 000)-derived peptide amino acids (aa) 3-13 (hsp65 aa 3-13) is recognized by the exclusive usage of the TRBV5 gene as well as a conserved CDR3 region in a tuberculoid leprosy patient. In the present study we analyzed the TcR of T-cell clones specific for hsp65 aa 3-13 derived from three healthy individuals with a response level similar to that of the leprosy patient. We show that unlike the tuberculoid leprosy patient, healthy high responders have a diverse T-cell response to hsp65 aa 3-13. However, a striking observation was made: even though high responders have a diverse specific TcR repertoire, TRBV5-expressing clones from two healthy individuals could be isolated that were nearly identical to a dominant clone in the tuberculoid leprosy patient. In conclusion, the data show that restriction of TcR specific for an antigen correlates with the presence of that antigen in disease. However, the preferred TcR can also be detected in healthy high responders. A natural infection in vivo, as with the tuberculoid leprosy patient, may be responsible for the observed trimming and preferential outgrowth of a certain TcR. |
Databáze: | OpenAIRE |
Externí odkaz: |