Highly fluorinated polymers with sulfonate, sulfamide and N ,N -diethylamino groups for the capillary electromigration separation of proteins and steroid hormones

Autor: Dzema Daria, Emelianov Gennady, Kartsova Liudmila
Rok vydání: 2017
Předmět:
Zdroj: Journal of Separation Science. 40:3335-3342
ISSN: 1615-9306
DOI: 10.1002/jssc.201700283
Popis: Highly fluorinated polymers are promising materials in separation methods due to combination of high chemical and thermally stability, hydro- and oleophobicity and weak intermolecular forces. However, application of these polymers in chromatography is limited because of their low solubility in aqueous-organic solvents. In our research, the highly fluorinated water soluble polymers with –SO3−N(Et)4+, –SO2NH2 and –N(Et)2 terminal groups were synthesized and applied as additives to the background electrolyte for the separation of steroid hormones and proteins by capillary electromigration methods. It is shown that highly fluorinated polymers can be used both as pseudo-stationary phases in electrokinetic chromatography for high separation efficiency (N ∼ 200 × 103) and selectivity (α∼1.1) of uncharged analytes (e.g., steroid hormones), and as dynamic modifiers of fused silica capillary walls. The highest separation efficiency (N ∼ 1 × 106) and selectivity (α∼1.3) of steroid hormones was achieved by combination of sodium dodecyl sulfate and fluoropolymer with sulfonate groups in background electrolyte with pH 2. Dynamic wall coatings based on fluoropolymer with –SO2NH2 (which are easier and faster to create and wash off) exhibit significantly higher separation efficiency and selectivity compared to capillary electrochromatography on capillary columns based on polymethacrylate polymers. This article is protected by copyright. All rights reserved
Databáze: OpenAIRE