Highly fluorinated polymers with sulfonate, sulfamide and N ,N -diethylamino groups for the capillary electromigration separation of proteins and steroid hormones
Autor: | Dzema Daria, Emelianov Gennady, Kartsova Liudmila |
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Rok vydání: | 2017 |
Předmět: |
Polymers
Inorganic chemistry Filtration and Separation 02 engineering and technology Electrolyte 01 natural sciences Micellar electrokinetic chromatography Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Animals Humans Organic chemistry Horses Micelles Chromatography Micellar Electrokinetic Capillary chemistry.chemical_classification Capillary electrochromatography 010401 analytical chemistry Proteins Sodium Dodecyl Sulfate Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences Fluorocarbon Polymers Sulfonate chemistry Fluoropolymer Steroids 0210 nano-technology Selectivity Chickens |
Zdroj: | Journal of Separation Science. 40:3335-3342 |
ISSN: | 1615-9306 |
DOI: | 10.1002/jssc.201700283 |
Popis: | Highly fluorinated polymers are promising materials in separation methods due to combination of high chemical and thermally stability, hydro- and oleophobicity and weak intermolecular forces. However, application of these polymers in chromatography is limited because of their low solubility in aqueous-organic solvents. In our research, the highly fluorinated water soluble polymers with –SO3−N(Et)4+, –SO2NH2 and –N(Et)2 terminal groups were synthesized and applied as additives to the background electrolyte for the separation of steroid hormones and proteins by capillary electromigration methods. It is shown that highly fluorinated polymers can be used both as pseudo-stationary phases in electrokinetic chromatography for high separation efficiency (N ∼ 200 × 103) and selectivity (α∼1.1) of uncharged analytes (e.g., steroid hormones), and as dynamic modifiers of fused silica capillary walls. The highest separation efficiency (N ∼ 1 × 106) and selectivity (α∼1.3) of steroid hormones was achieved by combination of sodium dodecyl sulfate and fluoropolymer with sulfonate groups in background electrolyte with pH 2. Dynamic wall coatings based on fluoropolymer with –SO2NH2 (which are easier and faster to create and wash off) exhibit significantly higher separation efficiency and selectivity compared to capillary electrochromatography on capillary columns based on polymethacrylate polymers. This article is protected by copyright. All rights reserved |
Databáze: | OpenAIRE |
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