Detection of apoptosis in acute promyelocytic leukemia cells in vivo during differentiation-induction with all-trans retinoic acid in combination with chemotherapy
| Autor: | Hoshiro Suzuki, Toshiaki Saito, Masue Imaizumi, Yutaka Hayashi, Atsushi Sato, Kazuie Iinuma, Miyako Yoshinari |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Acute promyelocytic leukemia Cancer Research Adolescent Cellular differentiation Retinoic acid Clone (cell biology) Apoptosis Tretinoin DNA Fragmentation Biology chemistry.chemical_compound Leukemia Promyelocytic Acute Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured medicine Humans Fragmentation (cell biology) Child neoplasms Cell Differentiation Hematology medicine.disease Oncology chemistry Cancer research DNA fragmentation Female medicine.drug |
| Zdroj: | Leukemia Research. 23:827-832 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/s0145-2126(99)00098-3 |
| Popis: | In two patients with acute promyelocytic leukemia (APL) treated with all-trans retinoic acid (ATRA) in combination with chemotherapy, we demonstrated that cells with apoptotic morphology were recognized in a small fraction of whole blood cells (0.2-0.4%) at the regression phase of leukocytosis with mostly maturing aberrant granulocytes of APL clone origin. Moreover, in a light-density cell fraction ( < 1.077) of peripheral blood or marrow cells obtained from three patients, DNA fragmentation was detected by agarose gel electrophoresis temporarily. These findings may suggest that, during the treatment with ATRA, a small fraction of APL cells maturing toward the stage of terminal differentiation underwent apoptosis in vivo, which might be enriched by the combination of chemotherapy to ATRA administration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect