Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma
| Autor: | L. Zanfini, Federica Menichini, F. Somma, Giancarlo Statti, Rosa Tundis, G. Menichini, Filomena Conforti, Eugenio Provenzano, Carmine Alfano |
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| Rok vydání: | 2012 |
| Předmět: |
FICUS-CARICA LEAVES
ANTIOXIDANT ACTIVITY PHENOLIC-COMPOUNDS FRUIT Ultraviolet Rays DPPH Linoleic acid Flavonoid Bergapten Antioxidants Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Cell Line Tumor Humans Melanoma chemistry.chemical_classification Plant Stems Traditional medicine biology Plant Extracts Fatty acid Original Articles Cell Biology General Medicine Plant Components Aerial Ficus biology.organism_classification Antineoplastic Agents Phytogenic Plant Leaves chemistry Phytochemical Biochemistry Polyphenol Plant Bark Lipid Peroxidation Carica |
| Zdroj: | Cell Proliferation. 45:279-285 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.2012.00816.x |
| Popis: | Objectives To date, Ficus carica L. cultivar Dottato (F. carica) has not been studied from a phototoxic point of view. In the present work, aerial components of F. carica from Italy, were examined to assess their antioxidant and phototoxic activity on human melanoma cells. A relationship between antioxidant, phototoxic activities and chemical composition has also been investigated. Materials and methods Coumarin and fatty acid content in F. carica leaves, bark and woody parts were examined and compared by capillary GC and GC/MS. Polyphenolic content was also determined. Linoleic acid peroxidation and DPPH test were used to assess antioxidant activities, and MTT assay was used to evaluate anti-proliferative activity, on C32 human melanoma cells, after irradiation with a UVA dose of 1.08 J/cm2. Results Leaves demonstrated the best antioxidant and anti-proliferative activity in comparison to bark and wood. In particular, leaves were shown to possess the highest anti-radical activity and inhibition of peroxidation, with IC50 values of 64 and 1.48 μg/ml respectively. The leaves had highest anti-proliferative activity with IC50 value of 3.92 μg/ml. The phytochemical investigation revealed different composition between the coumarins, psoralen and bergapten, fatty acids, polyphenols and flavonoid content among plant parts. Conclusions Data obtained indicate that this type of fig tree may constitute an excellent source of bioactive compounds, such as phenolics, coumarins and fatty acids. This study offers a new perspective in developing others formulations potentially useful in photodynamic therapy for treatment of non-melanoma skin cancers. |
| Databáze: | OpenAIRE |
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