Exploiting Amyloid Fibril Lamination for Nanotube Self-Assembly
| Autor: | David G. Lynn, Vincent P. Conticello, Kun Lu, Pappannan Thiyagarajan, Jaby Jacob |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Neutrons
Amyloid Nanotube Amyloid beta-Peptides Chemistry Atomic force microscopy Circular Dichroism X-Rays Nanotechnology General Chemistry Lamination (topology) Microscopy Atomic Force Fibril Amyloid fibril Biochemistry Peptide Fragments Catalysis Colloid and Surface Chemistry Scattering radiation Scattering Radiation Self-assembly |
| Zdroj: | Journal of the American Chemical Society. 125:6391-6393 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Fundamental questions about the relative arrangement of the beta-sheet arrays within amyloid fibrils remain central to both its structure and the mechanism of self-assembly. Recent computational analyses suggested that sheet-to-sheet lamination was limited by the length of the strand. On the basis of this hypothesis, a short seven-residue segment of the Alzheimer's disease-related Abeta peptide, Abeta(16-22), was allowed to self-assemble under conditions that maintained the basic amphiphilic character of Abeta. Indeed, the number increased over 20-fold to 130 laminates, giving homogeneous bilayer structures that supercoil into long robust nanotubes. Small-angle neutron scattering and X-ray scattering defined the outer and inner radii of the nanotubes in solution to contain a 44-nm inner cavity with 4-nm-thick walls. Atomic force microscopy and transmission electron microscopy images further confirmed these homogeneous arrays of solvent-filled nanotubes arising from a flat rectangular bilayer, 130 nm wide x 4 nm thick, with each bilayer leaflet composed of laminated beta-sheets. The corresponding backbone H-bonds are along the long axis, and beta-sheet lamination defines the 130-nm bilayer width. This bilayer coils to give the final nanotube. Such robust and persistent self-assembling nanotubes with positively charged surfaces of very different inner and outer curvature now offer a unique, robust, and easily accessible scaffold for nanotechnology. |
| Databáze: | OpenAIRE |
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