Quantification of phenolic acid metabolites in humans by LC-MS: a structural and targeted metabolomics approach
| Autor: | Rodolfo Bongiovanni, Isaac T. Schiefer, Mark E. Obrenovich, Curtis J. Donskey, George E. Jaskiw, Ling Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gastrointestinal microbiota Clinical Biochemistry General Medicine Phenolic acid Analytical Chemistry 03 medical and health sciences Medical Laboratory Technology chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Metabolomics Propanoic acid chemistry Biochemistry Isomerism Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Hydroxybenzoates Humans General Pharmacology Toxicology and Pharmaceutics 030217 neurology & neurosurgery Chromatography High Pressure Liquid Targeted metabolomics |
| Zdroj: | Bioanalysis. 10(19) |
| ISSN: | 1757-6199 |
| Popis: | Aim: Co-metabolism between a human host and the gastrointestinal microbiota generates many small phenolic molecules such as 3-hydroxy-3-(3-hydroxyphenyl)propanoic acid (3,3-HPHPA), which are reported to be elevated in schizophrenia and autism. Characterization of these chemicals, however, has been limited by analytic challenges. Methodology/results: We applied HPLC to separate and quantify over 50 analytes, including multiple structural isomers of 3,3-HPHPA in human cerebrospinal fluid, serum and urine. Confirmation of identity was provided by NMR, by MS and other detection methods. The highly selective methods support rapid quantification of multiple metabolites and exhibit superior chromatographic behavior. Conclusion: An improved ultra-HPLC–MS/MS and structural approaches can accurately quantify 3,3-HPHPA and related analytes in human biological matrices. |
| Databáze: | OpenAIRE |
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