Synthesis, characterization, and DNA binding, photocleavage, cytotoxicity, cellular uptake, apoptosis, and on–off light switching studies of Ru(II) mixed-ligand complexes containing 7-fluorodipyrido[3,2-a:2′,3′-c]phenazine
| Autor: | Avudoddi Srishailam, Putta Venkat Reddy, Yata Praveen Kumar, N. Deepika, Sirasani Satyanarayana, Chittimalli Shobha Devi |
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| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
Light Stereochemistry Phenazine Intercalation (chemistry) chemistry.chemical_element Antineoplastic Agents Apoptosis Chemistry Techniques Synthetic Photochemistry Biochemistry Ruthenium Flow cytometry Inorganic Chemistry HeLa chemistry.chemical_compound Anti-Infective Agents Bromide Cell Line Tumor Escherichia coli Organometallic Compounds medicine Humans DNA Cleavage Cytotoxicity Cell Proliferation biology medicine.diagnostic_test Viscosity Biological Transport DNA biology.organism_classification chemistry Phenazines |
| Zdroj: | JBIC Journal of Biological Inorganic Chemistry. 18:751-766 |
| ISSN: | 1432-1327 0949-8257 |
| Popis: | Four new ruthenium(II) polypyridyl complexes-[Ru(phen)2(7-F-dppz)](2+) (7-F-dppz is 7-fluorodipyrido[3,2-a:2',3'-c]phenazine, phen is 1,10-phenanthroline), [Ru(bpy)2(7-F-dppz)](2+)(2) (bpy is 2,2'-bipyridine), [Ru(dmb)2(7-F-dppz)](2+) (dmb is 4,4'-dimethyl-2,2'-bipyridine), and [Ru(hdpa)2(7-F-dppz)](2+) (hdpa is 2,2'-dipyridylamine)-have been synthesized and characterized. Their DNA binding behavior has been explored by various spectroscopic titrations and viscosity measurements, which indicated that all the complexes bind to calf thymus DNA by means of intercalation with different binding strengths. The light switching properties of these complexes have been evaluated, and their antimicrobial activities have been investigated. Photoinduced DNA cleavage studies have been performed. All the complexes exhibited efficient photocleavage of pBR322 DNA on irradiation. The cytotoxicity of these complexes has been evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay with various tumor cell lines. Cellular uptake was studied by flow cytometry and confocal microscopy. Flow cytometry experiments showed that these complexes induced apoptosis of HeLa cell lines. |
| Databáze: | OpenAIRE |
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