Stereoisomer-specific inhibition of superoxide anion-induced rat aortic smooth-muscle cell proliferation by 17beta-estradiol is estrogen receptor dependent
| Autor: | Mark C. Lavigne, Mann Y. Leong-Son, Thais Alibadi, Peter W. Ramwell, Sam Cathapermal |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Xanthine Oxidase medicine.drug_class Polyunsaturated Alkamides Estrogen receptor Aorta Thoracic Biology Muscle Smooth Vascular Superoxide dismutase Rats Sprague-Dawley chemistry.chemical_compound Superoxides Internal medicine medicine Animals Testosterone Xanthine oxidase Cells Cultured Progesterone Pharmacology Dose-Response Relationship Drug Estradiol Cell growth Superoxide Estrogen Antagonists Stereoisomerism Xanthine Rats Endocrinology chemistry Receptors Estrogen Estrogen biology.protein Cardiology and Cardiovascular Medicine Thymidine Cell Division |
| Zdroj: | Journal of cardiovascular pharmacology. 31(4) |
| ISSN: | 0160-2446 |
| Popis: | An in vitro xanthine/xanthine oxidase reaction system was used to generate superoxide anions that significantly stimulated tritiated [3H]thymidine incorporation into endothelium-removed (denuded) male rat aortic explants. Tritiated thymidine uptake was used as an index of vascular smooth-muscle cell (VSMC) proliferation. Superoxide dismutase (SOD) significantly attenuated the oxygen free radical-induced proliferative response of these cells. 17Beta-estradiol (17beta-E) significantly inhibited superoxide anion-induced VSMC proliferation. In contrast, the growth-modifying effects of 17beta-E were not mimicked by 17alpha-estradiol (17alpha-E), progesterone, or testosterone. The pure estrogen receptor (ER) antagonist, ICI 164,384, reversed the growth-inhibitory effect of 17beta-E. 17Beta-estradiol failed directly to reduce in vitro superoxide anion production or to modify xanthine oxidase activity. Therefore, these data indicate that 17beta-E, through an ER-dependent mechanism, specifically and significantly inhibited superoxide anion-mediated SMC proliferation in denuded rat aortic explants. |
| Databáze: | OpenAIRE |
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