Knockdown of Mitogen-Activated Protein Kinase Kinase 3 Negatively Regulates Hepatitis A Virus Replication

Autor: Mitsuhiko Moriyama, Hiroaki Okamoto, Ryota Masuzaki, Naoki Matsumoto, Reina Sasaki-Tanaka, Tatsuo Kanda
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Carcinoma
Hepatocellular
QH301-705.5
MAP Kinase Kinase 3
viruses
Biology
Mitogen-activated protein kinase kinase
Virus Replication
zinc chloride
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
0302 clinical medicine
Chlorides
Downregulation and upregulation
Toll-like receptor
Tumor Cells
Cultured
Humans
Biology (General)
Physical and Theoretical Chemistry
Protein kinase A
Receptor
QD1-999
Molecular Biology
Spectroscopy
Gene knockdown
Kinase
Liver Neoplasms
Organic Chemistry
virus diseases
General Medicine
Hepatitis A
Virology
digestive system diseases
Computer Science Applications
HAV
Chemistry
030104 developmental biology
MAP2K3
Zinc Compounds
030220 oncology & carcinogenesis
Host-Pathogen Interactions
Hepatocytes
Hepatitis A virus
Signal transduction
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 14
International Journal of Molecular Sciences, Vol 22, Iss 7420, p 7420 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22147420
Popis: Zinc chloride is known to be effective in combatting hepatitis A virus (HAV) infection, and zinc ions seem to be especially involved in Toll-like receptor (TLR) signaling pathways. In the present study, we examined this involvement in human hepatoma cell lines using a human TLR signaling target RT-PCR array. We also observed that zinc chloride inhibited mitogen-activated protein kinase kinase 3 (MAP2K3) expression, which could downregulate HAV replication in human hepatocytes. It is possible that zinc chloride may inhibit HAV replication in association with its inhibition of MAP2K3. In that regard, this study set out to determine whether MAP2K3 could be considered a modulating factor in the development of the HAV pathogen-associated molecular pattern (PAMP) and its triggering of interferon-β production. Because MAP2K3 seems to play a role in antiviral immunity against HAV infection, it is a promising target for drug development. The inhibition of MAP2K3 may also prevent HAV patients from developing a severe hepatitis A infection.
Databáze: OpenAIRE
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