Isolation of Flavonoids From Wild Aquilaria sinensis Leaves by an Improved Preparative High-Speed Counter-Current Chromatography Apparatus
Autor: | Hai-Guang Gong, Liang Yaoguang, Yang Maoxun, Yoichiro Ito, Tianyou Zhang, Yong-Fang Huang, He-Ru Chen |
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Rok vydání: | 2017 |
Předmět: |
Cell Survival
Antineoplastic Agents Aquilaria sinensis 01 natural sciences High-performance liquid chromatography Article Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Countercurrent chromatography Aquilaria Humans Countercurrent Distribution Flavonoids Chromatography biology 010405 organic chemistry Hep G2 Cells General Medicine biology.organism_classification 0104 chemical sciences Plant Leaves chemistry Thymelaeaceae 030220 oncology & carcinogenesis Genkwanin Kaempferol Quercetin |
Zdroj: | Journal of Chromatographic Science. 56:18-24 |
ISSN: | 1945-239X 0021-9665 |
Popis: | Four flavonoids including apigenin-7,4'-dimethylether, genkwanin, quercetin, and kaempferol were isolated in a preparative or semi-preparative scale from the leaves of wild Aquilaria sinensis using an improved preparative high-speed counter-current chromatography apparatus. The separations were performed with a two-phase solvent system composed of hexane-ethyl acetate, methanol-water at suitable volume ratios. The obtained fractions were analyzed by HPLC, and the identification of each target compound was carried out by ESI-MS and NMR. The yields of the above four target flavonoids were 4.7, 10.0, 11.0 and 4.4%, respectively. All these four flavonoids exhibited nitrite scavenging activities with the clearance rate of 12.40 ± 0.20%, 5.84 ± 0.03%, 28.10 ± 0.17% and 5.19 ± 0.11%, respectively. Quercetin was originally isolated from the Thymelaeaceae family, while kaempferol was isolated from the Aquilaria genus for the first time. In cytotoxicity test these two flavonoids exhibited moderate inhibitory activities against HepG2 cells with the IC50 values of 12.54 ± 1.37 and 38.63 ± 4.05 μM, respectively. |
Databáze: | OpenAIRE |
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