Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegans
| Autor: | Esther Neufeld, Benjamin Podbilewicz, Yosef Gruenbaum, Naomi Feinstein, Ayelet Margalit, Katherine L. Wilson |
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| Rok vydání: | 2007 |
| Předmět: |
Nuclear Envelope
Barrier-to-autointegration factor Emerin Biology Article Cell Movement medicine Animals Humans Caenorhabditis elegans Caenorhabditis elegans Proteins Research Articles Genetics Cell fusion Muscles Cell Biology biology.organism_classification Muscular Dystrophy Emery-Dreifuss Chromatin Cell biology Cell nucleus Histone medicine.anatomical_structure Epidermal Cells biology.protein Epidermis Carrier Proteins Lamin |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Barrier to autointegration factor (BAF) binds double-stranded DNA, selected histones, transcription regulators, lamins, and LAP2–emerin–MAN1 (LEM) domain proteins. During early Caenorhabditis elegans embryogenesis, BAF-1 is required to organize chromatin, capture segregated chromosomes within the nascent nuclear envelope, and assemble lamin and LEM domain proteins in reforming nuclei. In this study, we used C. elegans with a homozygous deletion of the baf-1 gene, which survives embryogenesis and larval stages, to report that BAF-1 regulates maturation and survival of the germline, cell migration, vulva formation, and the timing of seam cell fusion. In the seam cells, BAF-1 represses the expression of the EFF-1 fusogen protein, but fusion still occurs in C. elegans lacking both baf-1 and eff-1. This suggests the existence of an eff-1–independent mechanism for cell fusion. BAF-1 is also required to maintain the integrity of specific body wall muscles in adult animals, directly implicating BAF in the mechanism of human muscular dystrophies (laminopathies) caused by mutations in the BAF-binding proteins emerin and lamin A. |
| Databáze: | OpenAIRE |
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