A novel angiotensin I-converting enzyme mutation (S333W) impairs N-domain enzymatic cleavage of the anti-fibrotic peptide, AcSDKP
| Autor: | David E. Schwartz, Edward D. Sturrock, Kyle Hogarth, Ross G. Douglas, Sylva L. U. Schwager, Sergei M. Danilov, Isolda A. Popova, Michael S. Wade, Andrew B. Nesterovitch, Joe G.N. Garcia, Nakul Bhardwaj |
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| Přispěvatelé: | Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
ACE inhibitors
Substitution mutation Mutant lcsh:Medicine Peptide Cardiovascular Biochemistry Membrane proteins lcsh:Science Peptide sequence chemistry.chemical_classification Multidisciplinary biology Immunochemistry Hydrolysis CHO cells Enzymes Mutation detection Blood Medicine Oligopeptides Research Article Molecular Sequence Data Peptidyl-Dipeptidase A Cell Line Cricetulus Genetic Mutation Renin–angiotensin system Genetics Animals Humans Amino Acid Sequence Biology Enzyme Kinetics Clinical Genetics Tetrapeptide Point mutation Crystal structure lcsh:R Personalized Medicine Active site Proteins Molecular biology Fibrosis Kinetics Enzyme Metabolism chemistry Mutation Enzyme Structure biology.protein lcsh:Q Peptides Sequence Alignment |
| Zdroj: | PLoS One PLoS ONE PLoS ONE, Vol 9, Iss 2, p e88001 (2014) |
| DOI: | 10.1371/journal.pone.0088001 |
| Popis: | Background Angiotensin I-converting enzyme (ACE) has two functional N- and C-domain active centers that display differences in the metabolism of biologically-active peptides including the hemoregulatory tetrapeptide, Ac-SDKP, hydrolysed preferentially by the N domain active center. Elevated Ac-SDKP concentrations are associated with reduced tissue fibrosis. Results We identified a patient of African descent exhibiting unusual blood ACE kinetics with reduced relative hydrolysis of two synthetic ACE substrates (ZPHL/HHL ratio) suggestive of the ACE N domain center inactivation. Inhibition of blood ACE activity by anti-catalytic mAbs and ACE inhibitors and conformational fingerprint of blood ACE suggested overall conformational changes in the ACE molecule and sequencing identified Ser333Trp substitution in the N domain of ACE. In silico analysis demonstrated S333W localized in the S1 pocket of the active site of the N domain with the bulky Trp adversely affecting binding of ACE substrates due to steric hindrance. Expression of mutant ACE (S333W) in CHO cells confirmed altered kinetic properties of mutant ACE and conformational changes in the N domain. Further, the S333W mutant displayed decreased ability (5-fold) to cleave the physiological substrate AcSDKP compared to wild-type ACE. Conclusions and Significance A novel Ser333Trp ACE mutation results in dramatic changes in ACE kinetic properties and lowered clearance of Ac-SDKP. Individuals with this mutation (likely with significantly increased levels of the hemoregulatory tetrapeptide in blood and tissues), may confer protection against fibrosis. |
| Databáze: | OpenAIRE |
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