Synchronous detection of vascular tension and nitric oxide release in pulmonary artery: A combined application of confocal wire myograph with confocal laser scanning microscopy
| Autor: | Jie-Ling Zhu, Su-Mei Lai, Xiao-Ling Zhuang, Zhuang-Li Zhu, Long-Xin Gui, Mo-Jun Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Time Factors Confocal Vasodilator Agents 030204 cardiovascular system & hematology Pulmonary Artery Nitric Oxide Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.artery Confocal laser scanning microscopy medicine Animals Radiology Nuclear Medicine and imaging Enzyme Inhibitors Mice Inbred ICR Microscopy Confocal Dose-Response Relationship Drug business.industry Tension (physics) Myography General Medicine Synchronous detection Acetylcholine Vasodilation NG-Nitroarginine Methyl Ester chemistry Pulmonary artery Surgery Endothelium Vascular Nitric Oxide Synthase Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Biomedical engineering Myograph |
| Zdroj: | Vascular. 28(5) |
| ISSN: | 1708-539X |
| Popis: | Objectives To detect the vascular tension and nitric oxide (NO) release synchronously in mice pulmonary artery, we perform two experiments and present a novel application of confocal wire myograph coupled with the confocal laser scanning microscopy. Methods In the first experiment, viable endothelium-intact mouse pulmonary artery (outer diameter 100–300 μM) rings underwent a one-hour preincubation with a NO-specific fluorescent dye, 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate Calbiochem (2.5 μM), and then precontracted with phenylephrine (Phen, 10−6 M), and subsequently dilated in acetylcholine (ACh, 10−6 M – 10−4 M). The endothelium-dependent vasorelaxation and NO generation in pulmonary artery rings were simultaneously recorded. In the second experiment, after 30-min incubation with the former NO fluorescent dye, the qualified pulmonary artery rings were co-incubated for another 30 min with a nitric oxide synthase inhibitor, 10−4 M Nω-nitro-L-arginine-methyl-ester (L-NAME), and then pretreated with Phen (10−6 M) followed by ACh (10−5 M). The Ach-induced vasodilation and NO release were recorded simultaneously. Results ACh (10−6 M – 10−4 M) promoted pulmonary artery relaxation and intracellular NO release in a dose-dependent manner. Additionally, L-NAME (10−4 M) significantly attenuated the vasodilatation and the intracellular NO release. Conclusions This combined application visually confirms that the synchronous changes in Ach induced vasodilation and NO release, which provides a new method for cardiovascular research. |
| Databáze: | OpenAIRE |
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