First Isolation of Mimivirus in a Patient With Pneumonia
| Autor: | Didier Raoult, Isabelle Pagnier, Bernard La Scola, Hanene Saadi, Saïd Azza, Jouda Cherif, Mondher Boughalmi, Catherine Robert, Philippe Colson, Nicholas Armstrong, Majed Beji, Ghislain Fournous |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Marseilleviridae Molecular Sequence Data Pneumonia Viral Sequence Homology Acanthamoeba Genome Viral Microbiology Viral Proteins Cluster Analysis Humans Electrophoresis Gel Two-Dimensional Giant Virus Mimiviridae Serotyping Phylogeny Aged Mimivirus biology Pandoravirus Virophage Sequence Analysis DNA biology.organism_classification Virology DNA Virus Infections Microscopy Electron Infectious Diseases DNA Viral Female Radiography Thoracic Megavirus |
| Zdroj: | Clinical Infectious Diseases. 57:e127-e134 |
| ISSN: | 1537-6591 1058-4838 |
| DOI: | 10.1093/cid/cit354 |
| Popis: | BACKGROUND Mimiviridae Mimivirus, including the largest known viruses, multiply in amoebae. Mimiviruses have been linked to pneumonia, but they have never been isolated from patients. To further understand the pathogenic role of these viruses, we aimed to isolate them from a patient presenting with pneumonia. METHODS We cultured, on Acanthamoeba polyphaga amoebae, pulmonary samples from 196 Tunisian patients with community-acquired pneumonia during the period 2009-2010. An improved technique was used for Mimivirus isolation, which used agar plates where the growth of giant viruses is revealed by the formation of lysis plaques. Mimivirus serology was tested by microimmunofluorescence and by bidimensional immunoproteomic analysis using Mimivirus strains, to identify specific immunoreactive proteins. The new Mimivirus strain genome sequencing was performed on Roche 454 GS FLX Titanium, then AB SOLiD instruments. RESULTS We successfully isolated a Mimivirus (LBA111), the largest virus ever isolated in a human sample, from a 72-year-old woman presenting with pneumonia. Electron microscopy revealed a Mimivirus-like virion with a size of 554 ± 10 nm. The LBA111 genome is 1.23 megabases, and it is closely related to that of Megavirus chilensis. Furthermore, the serum from the patient reacted specifically to the virus compared to controls. CONCLUSIONS This is the first Mimivirus isolated from a human specimen. The findings presented above together with previous works establish that mimiviruses can be associated with pneumonia. The common occurrence of these viruses in water and soil makes them probable global agents that are worthy of investigation. |
| Databáze: | OpenAIRE |
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