Molecular identification and gene expression profiles of the T cell receptors and co-receptors in developing red-tailed phascogale (Phascogale calura) pouch young
| Autor: | Casey R. Borthwick, Julie M. Old, Lauren J. Young |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
White pulp T cell Immunology Population Receptors Antigen T-Cell Spleen Thymus Gland Phascogale 03 medical and health sciences medicine Animals RNA Messenger education Molecular Biology education.field_of_study Thymic involution biology Gene Expression Profiling Gene Expression Regulation Developmental biology.organism_classification Molecular biology 030104 developmental biology medicine.anatomical_structure Marsupialia Phascogale calura Pouch |
| Zdroj: | Molecular immunology. 101 |
| ISSN: | 1872-9142 |
| Popis: | Expressed coding sequences were identified for the T cell receptors TCRα, TCRβ, TCRγ, TCRδ, TCRμ, and co-receptors CD3e, CD4, and CD8α in the red-tailed phascogale (Phascogale calura). Expression of these genes was examined in the developing thymus and spleen of pouch young and juvenile individuals to detect any changes in expressionrelative to developmental stages. Transcripts of CD3e, CD4, TCRδ and TCRμ were first detected in the thorax at 1 day postpartum (dpp), and transcripts of all target genes were detected in the thymus from 3 dpp, and the spleen from 17 dpp. The relative expression of all target genes changed significantly over the course of pouch life and was associated with histological milestones of both the thymus and the spleen. Peak gene expression was observed early in pouch life in the thymus, with a decrease in expression associated with timing of thymic involution, while expression peaked later in the spleen and was associated with the initial differentiation of the red and white pulp and a population of T cells forming the periarterial lymphatic sheath (PALS). Active expression of these target genes within the thymus and spleen indicates that the young marsupials are actively developing immunocompetence during pouch life. |
| Databáze: | OpenAIRE |
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