Identification of Candidate Anthocyanin-Related Genes by Transcriptomic Analysis of ‘Furongli’ Plum (Prunus salicina Lindl.) during Fruit Ripening Using RNA-Seq
| Autor: | Fang Zhizhen, Ye Xinfu, Zhou Danrong, Jiang Cuicui, Pan Shaolin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Prunus salicina anthocyanin biosynthesis fruit ripening RNA-Seq Plant Science lcsh:Plant culture 01 natural sciences Prunus salicina Lindl Transcriptome 03 medical and health sciences Botany MYB lcsh:SB1-1110 KEGG Gene transcription factor Original Research Genetics biosynthetic enzyme biology Structural gene food and beverages Ripening biology.organism_classification 030104 developmental biology transcriptome 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 7 (2016) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2016.01338 |
| Popis: | Anthocyanins are important pigments and are responsible for red coloration in plums. However, little is known about the molecular mechanisms underlying anthocyanin accumulation in plum fruits. In this study, the RNA-seq technique was used to analyze the transcriptomic changes during fruit ripening in the red-fleshed plum (Prunus salicina Lindl.) cultivar ‘Furongli’. Over 161 million high-quality reads were assembled into 52,093 unigenes and 49.4% of these were annotated using public databases. Of these, 25,681 unigenes had significant hits to the sequences in the NCBI Nr database, 17,203 unigenes showed significant similarity to known proteins in the Swiss-Prot database and 5,816 and 8,585 unigenes had significant similarity to existing sequences in the Kyoto Encyclopedia of Genes and Genomes and the Cluster of Orthologous Groups databases, respectively. A total of 3,548 unigenes were differentially expressed during fruit ripening and 119 of these were annotated as involved in ‘biosynthesis of other secondary metabolites’. Biological pathway analysis and gene ontology term enrichment analysis revealed that 13 differentially expressed genes are involved in anthocyanin biosynthesis. Furthermore, transcription factors such as MYB and bHLH, which may control anthocyanin biosynthesis, were identified through coexpression analysis of transcription factors and structural genes. Real-time qPCR analysis of candidate genes showed good correlation with the transcriptome data. These results contribute to our understanding of the molecular mechanisms underlying anthocyanin biosynthesis in plum flesh. The transcriptomic data generated in this study provide a basis for further studies of fruit ripening in plum. |
| Databáze: | OpenAIRE |
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