The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells
| Autor: | Jana Brejchova, Kristina Cechova, Vladimir Holan, Jonas Kaufman, Lenka Roubalova, Petr Svoboda, Hana Ujcikova |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Proteomics Proteome Immunology Enolase High resolution Stimulation Lymphocyte Activation 03 medical and health sciences 0302 clinical medicine medicine Concanavalin A Immunology and Allergy Animals Lymphocytes Rats Wistar biology Morphine Chemistry Gene Expression Profiling Protein composition Molecular biology Myelin basic protein Rats 030104 developmental biology Neurology Gene Expression Regulation biology.protein Rat Spleen Neurology (clinical) 030217 neurology & neurosurgery Spleen medicine.drug |
| Zdroj: | Journal of neuroimmunology. 341 |
| ISSN: | 1872-8421 |
| Popis: | Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins; 101 proteins were down-regulated (>2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (>2-fold). |
| Databáze: | OpenAIRE |
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