Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1
Autor: | Shun'ichi Kuroda, Katsuyuki Tanizawa, Toshihide Okajima, Junko Ikuta, Kenji Tatematsu, Toshitsugu Fujita, Juri Hamada |
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Rok vydání: | 2004 |
Předmět: |
Axonal fasciculation
DNA Complementary Molecular Sequence Data Biophysics Nerve Tissue Proteins In situ hybridization Biology PC12 Cells Biochemistry Fasciculation Mice medicine Animals Humans Genes Tumor Suppressor Tissue Distribution Amino Acid Sequence RNA Messenger Northern blot Caenorhabditis elegans Proteins Molecular Biology Protein Kinase C Protein kinase C Adaptor Proteins Signal Transducing Neurons Messenger RNA Microscopy Confocal Sequence Homology Amino Acid Tumor Suppressor Proteins Neuropeptides Neurogenesis Cell Biology Blotting Northern Precipitin Tests Molecular biology Rats DNA-Binding Proteins Alternative Splicing COS Cells medicine.symptom Cell Adhesion Molecules FEZ1 Protein Binding |
Zdroj: | Biochemical and Biophysical Research Communications. 313:738-744 |
ISSN: | 0006-291X |
Popis: | Fasciculation and elongation protein zeta-1 (FEZ1) is a mammalian orthologue of the Caenorhabditis elegans UNC-76 protein involved in the axonal outgrowth and fasciculation and promotes neurite extension of PC12 cells through interaction with protein kinase C zeta (PKCzeta). The gene coding for FEZ2, a homologue of FEZ1, has also been reported in rat and human. In this study, we compared mRNA expression of FEZ1 and FEZ2 in adult rat tissues and mouse embryos by Northern blot and in situ hybridization analyses. In contrast to FEZ1 whose mRNA is expressed almost exclusively in rat brain and temporarily around the neurogenesis stage of mouse embryos, the message for FEZ2 is detected weakly in most tissues and abundantly throughout the mouse embryonic stages. Similar to FEZ1, FEZ2 interacted with PKCzeta and induced neurite extension of PC12 cells when coexpressed with a constitutively active mutant of PKCzeta. These results suggest that FEZ2 plays an important role in the morphological changes of various cells by associating with PKCzeta in a tissue-non-specific manner. |
Databáze: | OpenAIRE |
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