Expression of the Novel Epithelial Ca2+Channel ECaC1 in Rat Pancreatic Islets
| Autor: | Joost G. J. Hoenderop, René J. M. Bindels, Gerard J.M. Martens, Fred C.G.J. Sweep, Ad R. M. M. Hermus, Susan W. J. Janssen |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
0301 basic medicine Cell Membrane Permeability Patch-Clamp Techniques Physiology medicine.medical_treatment Regulation of salt and water reabsorption in the renal collecting duct Fluorescent Antibody Technique Kidney General (Non MeSH) Physiology General (Non MeSH) Kidney Concentrating Ability Endocrinology Adrenal Cortex Hormones Urinary Tract Cells Cultured Chemical Endocrinology Cultured Hypothalamic Hormones Reverse Transcriptase Polymerase Chain Reaction Water-Electrolyte Balance medicine.anatomical_structure Regulatie water en zouttransport in de verzamelbuis van de nier Immunohistochemistry Kidney Diseases Anatomy medicine.medical_specialty Histology Cells TRPV Cation Channels Urogenital System Biology Glucagon Cell Physiology (Non MeSH) Islets of Langerhans 03 medical and health sciences Calcitriol Internal medicine medicine Animals RNA Messenger Ion Transport 030102 biochemistry & molecular biology Binding protein Pancreatic islets Insulin Apical membrane Hormones Rats Rats Zucker 030104 developmental biology Diabetes Mellitus Type 2 Calcium Channels Homeostasis Hormone |
| Zdroj: | Journal of Histochemistry and Cytochemistry, 50, 6, pp. 789-798 Journal of Histochemistry and Cytochemistry, 50, 789-798 Journal of Histochemistry and Cytochemistry, 50, 789-98 Journal of Histochemistry and Cytochemistry, 50, 6, pp. 789-98 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/002215540205000605 |
| Popis: | The epithelial Ca2+channel, ECaC1, is primarily expressed in the apical membrane of vitamin D-responsive tissues. This study characterizes for the first time the presence of this novel channel in pancreatic tissue by reverse transcriptase-polymerase chain reaction and immunohistochemistry. In addition, the expression of ECaC1 was investigated in an animal model for Type 2 diabetes mellitus, the Zucker diabetic fatty (ZDF) rat. Identical staining patterns for ECaC1 and insulin were observed, whereas no co-localization of ECaC1 with glucagon was found. ECaC1, insulin, and prohormone convertase 1 (a neuroendocrine endoprotease expressed in secretory granules) showed a similar punctate staining. ECaC1 co-localized with the Ca2+binding protein calbindin-D28K in the β-cells. Furthermore, in contrast to wild-type rats, in ZDF rats aging led to a progressive decrease in both insulin and ECaC1 staining. Plasma 1,25-dihydroxyvitamin D3levels were similar in both control and ZDF rats and decreased with aging. Taken together, our findings indicate that this novel Ca2+channel may play a role in the regulation of endocrine Ca2+homeostasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|