RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts
| Autor: | Cathrin Gräwe, Suzan Stelloo, Michiel Vermeulen, Femke A.H. van Hout |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Proteome Proteomics and Chromatin Biology RNA Computational Biology RNA-Binding Proteins Bioengineering 02 engineering and technology Computational biology Biology 021001 nanoscience & nanotechnology Interactome 03 medical and health sciences 030104 developmental biology Clustered Regularly Interspaced Short Palindromic Repeats 0210 nano-technology Biotechnology |
| Zdroj: | Trends in Biotechnology |
| ISSN: | 0167-7799 |
| DOI: | 10.1016/j.tibtech.2020.11.011 |
| Popis: | RNA–protein interactions play an important role in numerous cellular processes in health and disease. In recent years, the global RNA-bound proteome has been extensively studied, uncovering many previously unknown RNA-binding proteins. However, little is known about which particular proteins bind to which specific RNA transcript. In this review, we provide an overview of methods to identify RNA–protein interactions, with a particular focus on strategies that provide insights into the interactome of specific RNA transcripts. Finally, we discuss challenges and future directions, including the potential of CRISPR-RNA targeting systems to investigate endogenous RNA–protein interactions. |
| Databáze: | OpenAIRE |
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